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Title: | Nascent pectin formed in Golgi apparatus of pea epicotyls by addition of uronic acids has different properties from nascent pectin at the stage of galactan elongation | Authors: | Abdel-Massih, Roula Rizkallah, Hind D. Saif Al-Din, Rabih Baydoun, Elias A-H. Brett, Cristopher T. |
Affiliations: | Department of Biology | Keywords: | Pisum sativum Polygalacturonan Xyloglucan |
Subjects: | Pectin Proteins |
Issue Date: | 2007 | Part of: | Journal of plant physiology | Volume: | 122 | Issue: | 1 | Start page: | 1 | End page: | 10 | Abstract: | Microsomal membranes were prepared from etiolated pea (Pisum sativum L.) epicotyls and used to form nascent [Uronic acid-14C]pectin. The enzyme products were characterized by selective enzymic degradation, gel permeation chromatography and analysis of cellulose binding properties. The product obtained had a molecular weight of around 40 kDa, which was significantly lower than that of nascent [Gal-14C]pectin prepared from the same tissues. It is composed mainly of polygalacturonan and perhaps also rhamnogalacturonan (RG-I). Evidence was obtained for the presence of a protein attached to the nascent [Uronic acid-14C]pectin, but it was unaffected by endoglucanase and did not bind to cellulose. Hence, no xyloglucan appeared to be attached to the nascent [Uronic acid-14C]pectin. A model is proposed in which xyloglucan is attached to nascent pectin after formation of homogalacturonan, but before the pectin leaves the Golgi apparatus. |
URI: | https://scholarhub.balamand.edu.lb/handle/uob/2296 | DOI: | 10.1016/j.jplph.2006.06.013 | Ezproxy URL: | Link to full text | Type: | Journal Article |
Appears in Collections: | Department of Biology |
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