Please use this identifier to cite or link to this item:
Title: Nascent pectin formed in Golgi apparatus of pea epicotyls by addition of uronic acids has different properties from nascent pectin at the stage of galactan elongation
Authors: Abdel-Massih, Roula
Rizkallah, Hind D.
Saif Al-Din, Rabih
Baydoun, Elias A-H.
Brett, Cristopher T.
Affiliations: Department of Biology 
Keywords: Pisum sativum
Subjects: Pectin
Issue Date: 2007
Part of: Journal of plant physiology
Volume: 122
Issue: 1
Start page: 1
End page: 10
Microsomal membranes were prepared from etiolated pea (Pisum sativum L.) epicotyls and used to form nascent [Uronic acid-14C]pectin. The enzyme products were characterized by selective enzymic degradation, gel permeation chromatography and analysis of cellulose binding properties. The product obtained had a molecular weight of around 40 kDa, which was significantly lower than that of nascent [Gal-14C]pectin prepared from the same tissues. It is composed mainly of polygalacturonan and perhaps also rhamnogalacturonan (RG-I). Evidence was obtained for the presence of a protein attached to the nascent [Uronic acid-14C]pectin, but it was unaffected by endoglucanase and did not bind to cellulose. Hence, no xyloglucan appeared to be attached to the nascent [Uronic acid-14C]pectin. A model is proposed in which xyloglucan is attached to nascent pectin after formation of homogalacturonan, but before the pectin leaves the Golgi apparatus.
DOI: 10.1016/j.jplph.2006.06.013
Ezproxy URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Biology

Show full item record


checked on Oct 16, 2021

Record view(s)

checked on Oct 18, 2021

Google ScholarTM


Dimensions Altmetric

Dimensions Altmetric

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.