Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/2296
Title: Nascent pectin formed in Golgi apparatus of pea epicotyls by addition of uronic acids has different properties from nascent pectin at the stage of galactan elongation
Authors: Abdel-Massih, Roula
Rizkallah, Hind D.
Saif Al-Din, Rabih
Baydoun, Elias A-H.
Brett, Cristopher T.
Affiliations: Department of Biology 
Keywords: Pisum sativum
Polygalacturonan
Xyloglucan
Subjects: Pectin
Proteins
Issue Date: 2007
Part of: Journal of plant physiology
Volume: 122
Issue: 1
Start page: 1
End page: 10
Abstract: 
Microsomal membranes were prepared from etiolated pea (Pisum sativum L.) epicotyls and used to form nascent [Uronic acid-14C]pectin. The enzyme products were characterized by selective enzymic degradation, gel permeation chromatography and analysis of cellulose binding properties. The product obtained had a molecular weight of around 40 kDa, which was significantly lower than that of nascent [Gal-14C]pectin prepared from the same tissues. It is composed mainly of polygalacturonan and perhaps also rhamnogalacturonan (RG-I). Evidence was obtained for the presence of a protein attached to the nascent [Uronic acid-14C]pectin, but it was unaffected by endoglucanase and did not bind to cellulose. Hence, no xyloglucan appeared to be attached to the nascent [Uronic acid-14C]pectin. A model is proposed in which xyloglucan is attached to nascent pectin after formation of homogalacturonan, but before the pectin leaves the Golgi apparatus.
URI: https://scholarhub.balamand.edu.lb/handle/uob/2296
DOI: 10.1016/j.jplph.2006.06.013
Ezproxy URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Biology

Show full item record

SCOPUSTM   
Citations

5
checked on Oct 16, 2021

Record view(s)

4
checked on Oct 18, 2021

Google ScholarTM

Check

Dimensions Altmetric

Dimensions Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.