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|Title:||Nascent pectin formed in Golgi apparatus of pea epicotyls by addition of uronic acids has different properties from nascent pectin at the stage of galactan elongation||Authors:||Abdel-Massih, Roula
Rizkallah, Hind D.
Saif Al-Din, Rabih
Baydoun, Elias A-H.
Brett, Cristopher T.
|Affiliations:||Department of Biology||Keywords:||Pisum sativum
|Issue Date:||2007||Part of:||Journal of plant physiology||Volume:||122||Issue:||1||Start page:||1||End page:||10||Abstract:||
Microsomal membranes were prepared from etiolated pea (Pisum sativum L.) epicotyls and used to form nascent [Uronic acid-14C]pectin. The enzyme products were characterized by selective enzymic degradation, gel permeation chromatography and analysis of cellulose binding properties. The product obtained had a molecular weight of around 40 kDa, which was significantly lower than that of nascent [Gal-14C]pectin prepared from the same tissues. It is composed mainly of polygalacturonan and perhaps also rhamnogalacturonan (RG-I). Evidence was obtained for the presence of a protein attached to the nascent [Uronic acid-14C]pectin, but it was unaffected by endoglucanase and did not bind to cellulose. Hence, no xyloglucan appeared to be attached to the nascent [Uronic acid-14C]pectin. A model is proposed in which xyloglucan is attached to nascent pectin after formation of homogalacturonan, but before the pectin leaves the Golgi apparatus.
|URI:||https://scholarhub.balamand.edu.lb/handle/uob/2296||DOI:||10.1016/j.jplph.2006.06.013||Ezproxy URL:||Link to full text||Type:||Journal Article|
|Appears in Collections:||Department of Biology|
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