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https://scholarhub.balamand.edu.lb/handle/uob/2118| Title: | In vitro biosynthesis of 1,4-ß-galactan attached to a pectin-xyloglucan complex in pea | Authors: | Abdel-Massih, Roula Baydoun, Elias A-H. Brett, Cristopher T. |
Affiliations: | Department of Biology | Keywords: | Galactan Galactosyltransferase Pisum Xyloglucan |
Subjects: | Pectin | Issue Date: | 2003 | Part of: | Planta | Volume: | 216 | Issue: | 3 | Start page: | 502 | End page: | 511 | Abstract: | Particulate enzyme preparations were prepared from etiolated pea (Pisum sativum L.) epicotyls and used to assay for 1,4-β-galactan synthase using UDP-[U-14C]galactose. Optimum conditions for 1,4-β-galactan synthesis were determined. The enzyme products were characterized by selective enzymic degradation, gel permeation chromatography and anion-exchange chromatography. Evidence was obtained for the formation of 1,4-β-galactan chain attached to a pectic backbone containing both polygalacturonic acid and rhamnogalacturonan I. The results also indicated that part or all of this nascent pectin was present as a complex with xyloglucan. |
URI: | https://scholarhub.balamand.edu.lb/handle/uob/2118 | DOI: | 10.1007/s00425-002-0861-y | Ezproxy URL: | Link to full text | Type: | Journal Article |
| Appears in Collections: | Department of Biology |
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