Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/2118
Title: In vitro biosynthesis of 1,4-ß-galactan attached to a pectin-xyloglucan complex in pea
Authors: Abdel-Massih, Roula
Baydoun, Elias A-H.
Brett, Cristopher T.
Affiliations: Department of Biology 
Keywords: Galactan
Galactosyltransferase
Pisum
Xyloglucan
Subjects: Pectin
Issue Date: 2003
Part of: Planta
Volume: 216
Issue: 3
Start page: 502
End page: 511
Abstract: 
Particulate enzyme preparations were prepared from etiolated pea (Pisum sativum L.) epicotyls and used to assay for 1,4-β-galactan synthase using UDP-[U-14C]galactose. Optimum conditions for 1,4-β-galactan synthesis were determined. The enzyme products were characterized by selective enzymic degradation, gel permeation chromatography and anion-exchange chromatography. Evidence was obtained for the formation of 1,4-β-galactan chain attached to a pectic backbone containing both polygalacturonic acid and rhamnogalacturonan I. The results also indicated that part or all of this nascent pectin was present as a complex with xyloglucan.
URI: https://scholarhub.balamand.edu.lb/handle/uob/2118
DOI: 10.1007/s00425-002-0861-y
Ezproxy URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Biology

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