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|Title:||In vitro biosynthesis of 1,4-ß-galactan attached to a pectin-xyloglucan complex in pea||Authors:||Abdel-Massih, Roula
Baydoun, Elias A-H.
Brett, Cristopher T.
|Affiliations:||Department of Biology||Keywords:||Galactan
|Subjects:||Pectin||Issue Date:||2003||Part of:||Planta||Volume:||216||Issue:||3||Start page:||502||End page:||511||Abstract:||
Particulate enzyme preparations were prepared from etiolated pea (Pisum sativum L.) epicotyls and used to assay for 1,4-β-galactan synthase using UDP-[U-14C]galactose. Optimum conditions for 1,4-β-galactan synthesis were determined. The enzyme products were characterized by selective enzymic degradation, gel permeation chromatography and anion-exchange chromatography. Evidence was obtained for the formation of 1,4-β-galactan chain attached to a pectic backbone containing both polygalacturonic acid and rhamnogalacturonan I. The results also indicated that part or all of this nascent pectin was present as a complex with xyloglucan.
|URI:||https://scholarhub.balamand.edu.lb/handle/uob/2118||DOI:||10.1007/s00425-002-0861-y||Ezproxy URL:||Link to full text||Type:||Journal Article|
|Appears in Collections:||Department of Biology|
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