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Title: Phenotypic and molecular characterization of multi-drug resistant Klebsiella spp. isolates recovered from clinical settings
Authors: Diab, Hassan
Rahy, Kelven
Jisr, Tamima
Chaar, Mira El 
Abboud, Edmond
Tokajian, Sima
Affiliations: Department of Medical Laboratory Sciences 
Keywords: Klebsiella pneumoniae
Klebsiella variicola
Klebsiella quasipneumoniae
Issue Date: 2024-04
Publisher: Elsevier
Part of: Infection, Genetics and Evolution
Volume: 119
Klebsiella pneumoniae is a Gram-negative bacterium that colonizes the gastrointestinal tract and nasopharynx with many being linked to nosocomial infections. Extended-spectrum β-lactamases (ESBL)-producing and carbapenem-resistant K. pneumoniae is recognized by the World Health Organization (WHO) as a critical public health concern. In this study, whole-genome sequencing (WGS) - based analysis was performed to understand the molecular epidemiology of multi-drug resistant Klebsiella spp. clinical isolates. Genome comparison, multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and whole-genome-SNP-based phylogenetic analysis (wg-SNP) were used for in-depth molecular characterization. in silico typing was used to determine the resistance genes, virulence factors, Inc. groups, and capsular types. All except one isolate were non-susceptible to meropenem and 89% were non-susceptible to ertapenem and imipenem. blaNDM, blaOXA-48, and blaKPC were the detected carbapenemases with blaNDM-1 found in half of the sequenced genomes. Resistance to colistin was detected in one isolate and was linked to several genetic alterations in crrB, pmrB, and pmrC genes. The most common plasmid type was IncFIB followed by IncR, and the Type 3 fimbriae, encoded by the mrkABCDF operon, was conserved among all isolates. The most common sequence- (ST) and K-type detected were ST147 and K64. The prevelance and the genomic relatedness of ST147 isolates, as shown by the data from SNP-based phylogenetic analysis, PFGE, and genomic clustering, may be an outbreak marker. However, this can only be validated through a more comprehensive study encompassing a wider sampling scheme and over an extended timeframe.
ISSN: 15671348
DOI: 10.1016/j.meegid.2024.105583
Ezproxy URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Medical Laboratory Sciences

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