Please use this identifier to cite or link to this item:
https://scholarhub.balamand.edu.lb/handle/uob/7296
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Diab, Hassan | en_US |
dc.contributor.author | Rahy, Kelven | en_US |
dc.contributor.author | Jisr, Tamima | en_US |
dc.contributor.author | Chaar, Mira El | en_US |
dc.contributor.author | Abboud, Edmond | en_US |
dc.contributor.author | Tokajian, Sima | en_US |
dc.date.accessioned | 2024-03-27T09:47:05Z | - |
dc.date.available | 2024-03-27T09:47:05Z | - |
dc.date.issued | 2024-04 | - |
dc.identifier.issn | 15671348 | - |
dc.identifier.uri | https://scholarhub.balamand.edu.lb/handle/uob/7296 | - |
dc.description.abstract | Klebsiella pneumoniae is a Gram-negative bacterium that colonizes the gastrointestinal tract and nasopharynx with many being linked to nosocomial infections. Extended-spectrum β-lactamases (ESBL)-producing and carbapenem-resistant K. pneumoniae is recognized by the World Health Organization (WHO) as a critical public health concern. In this study, whole-genome sequencing (WGS) - based analysis was performed to understand the molecular epidemiology of multi-drug resistant Klebsiella spp. clinical isolates. Genome comparison, multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and whole-genome-SNP-based phylogenetic analysis (wg-SNP) were used for in-depth molecular characterization. in silico typing was used to determine the resistance genes, virulence factors, Inc. groups, and capsular types. All except one isolate were non-susceptible to meropenem and 89% were non-susceptible to ertapenem and imipenem. blaNDM, blaOXA-48, and blaKPC were the detected carbapenemases with blaNDM-1 found in half of the sequenced genomes. Resistance to colistin was detected in one isolate and was linked to several genetic alterations in crrB, pmrB, and pmrC genes. The most common plasmid type was IncFIB followed by IncR, and the Type 3 fimbriae, encoded by the mrkABCDF operon, was conserved among all isolates. The most common sequence- (ST) and K-type detected were ST147 and K64. The prevelance and the genomic relatedness of ST147 isolates, as shown by the data from SNP-based phylogenetic analysis, PFGE, and genomic clustering, may be an outbreak marker. However, this can only be validated through a more comprehensive study encompassing a wider sampling scheme and over an extended timeframe. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | Klebsiella pneumoniae | en_US |
dc.subject | Klebsiella variicola | en_US |
dc.subject | Klebsiella quasipneumoniae | en_US |
dc.subject | NDM | en_US |
dc.subject | ST147 | en_US |
dc.title | Phenotypic and molecular characterization of multi-drug resistant Klebsiella spp. isolates recovered from clinical settings | en_US |
dc.type | Journal Article | en_US |
dc.identifier.doi | 10.1016/j.meegid.2024.105583 | - |
dc.identifier.pmid | 38484958 | - |
dc.identifier.scopus | 2-s2.0-85187961537 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85187961537 | - |
dc.contributor.affiliation | Department of Medical Laboratory Sciences | en_US |
dc.description.volume | 119 | en_US |
dc.date.catalogued | 2024-03-27 | - |
dc.description.status | Published | en_US |
dc.identifier.ezproxyURL | http://ezsecureaccess.balamand.edu.lb/login?url=https://doi.org/10.1016/j.meegid.2024.105583 | en_US |
dc.relation.ispartoftext | Infection, Genetics and Evolution | en_US |
crisitem.author.parentorg | Faculty of Health Sciences | - |
Appears in Collections: | Department of Medical Laboratory Sciences |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.