Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/6328
Title: Multi-Step Biomass Fractionation of Grape Seeds from Pomace, a Zero-Waste Approach
Authors: Salem, Yara
Rajha, Hiba N
van den Broek, Lambertus A M
Safi, Carl
Togtema, Arnoud
Manconi, Maria
Manca, Maria Letizia
Debs, Esperance 
Hobaika, Zeina
Maroun, Richard G
Louka, Nicolas
Affiliations: Centre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-Alimentaire, Faculté des Sciences, Université Saint-Joseph de Beyrouth, Riad El Solh, P.O. Box 17-5208, Beirut 1104 2020, Lebanon
Ecole Supérieure d’Ingénieurs de Beyrouth (ESIB), Université Saint-Joseph de Beyrouth, CST Mkalles Mar Roukos, Riad El Solh, Beirut 1107 2050, Lebanon
Wageningen Food & Biobased Research, P.O. Box 17, 6700 AA Wageningen, The Netherlands
Wageningen Food & Biobased Research, P.O. Box 17, 6700 AA Wageningen, The Netherlands
Wageningen Food & Biobased Research, P.O. Box 17, 6700 AA Wageningen, The Netherlands
Centre for Nanobiotechnology Sardinia CNBS, Department of Scienze della Vita e dell’Ambiente, University of Cagliari, 09124 Cagliari, Italy
Centre for Nanobiotechnology Sardinia CNBS, Department of Scienze della Vita e dell’Ambiente, University of Cagliari, 09124 Cagliari, Italy
Faculty of Arts and Sciences 
Centre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-Alimentaire, Faculté des Sciences, Université Saint-Joseph de Beyrouth, Riad El Solh, P.O. Box 17-5208, Beirut 1104 2020, Lebanon
Centre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-Alimentaire, Faculté des Sciences, Université Saint-Joseph de Beyrouth, Riad El Solh, P.O. Box 17-5208, Beirut 1104 2020, Lebanon
Centre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-Alimentaire, Faculté des Sciences, Université Saint-Joseph de Beyrouth, Riad El Solh, P.O. Box 17-5208, Beirut 1104 2020, Lebanon
Keywords: Biorefinery
Grape byproducts valorization
Natural extracts
Issue Date: 2022-10-25
Publisher: MDPI
Part of: Plants
Volume: 11
Issue: 2831
Start page: 1
End page: 17
Abstract: 
Grape seeds are the wineries' main by-products, and their disposal causes ecological and environmental problems. In this study seeds from the pomace waste of autochthonous grape varieties from Lebanon, Obeidi (white variety) and Asswad Karech (red variety) were used for a multi-step biomass fractionation. For the first step, a lipid extraction was performed, and the obtained yield was 12.33% (w/w) for Obeidi and 13.04% (w/w) for Asswad Karech. For the second step, polyphenols' recovery from the defatted seeds was carried out, resulting in 12.0% (w/w) for Obeidi and 6.6% (w/w) for Asswad Karech, with Obeidi's extract having the highest total phenolic content (333.1 ± 1.6 mg GAE/g dry matter) and antioxidant activity (662.17 ± 0.01 µg/mL of Trolox equivalent). In the third step, the defatted and dephenolized seeds were subsequently extracted under alkaline conditions and the proteins were isoelectric precipitated. The recovered protein extract was 3.90% (w/w) for Obeidi and 4.11% (w/w) for Asswad Karech seeds, with Asswad Karech's extract having the highest protein content (64 ± 0.2 mg protein/g dry matter). The remaining exhausted residue can be valorized in cosmetic scrubs formulations as a replacement for plastic microbeads. The designed zero-waste approach multi-step biomass fractionation has the potential to improve the valorization of the side products (grape seeds) of these two Lebanese autochthonous grape varieties.
URI: https://scholarhub.balamand.edu.lb/handle/uob/6328
ISSN: 2223-7747
DOI: 10.3390/plants11212831
Open URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Biology

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