Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/2097
Title: Immunomodulatory effects of heat-killed Mycobacterium obuense on human blood dendritic cells
Authors: Bazzi, Samer 
Modjtahedi, Helmout
Mudan, Satvinder
Achkar, Marcel
Akle, Charles
Bahr, George M. 
Affiliations: Department of Biology 
Faculty of Medicine 
Keywords: Dendritic cells
Mycobacterium obuense
Immunomodulator
CD molecules
Toll-like receptors
Cytokines
Chemokines
Issue Date: 2017
Part of: Journal of innate immunity
Volume: 23
Issue: 7
Start page: 592
End page: 605
Abstract: 
Heat-killed (HK) Mycobacterium obuense is a novel immunomodulator, currently undergoing clinical evaluation as an immunotherapeutic agent in the treatment of cancer. Here, we examined the effect of in vitro exposure to HK M. obuense on the expression of different categories of surface receptors on human blood myeloid (m) and plasmacytoid (p) DCs. Moreover, we have characterized the cytokine and chemokine secretion patterns of purified total blood DCs stimulated with HK M. obuense. HK M. obuense significantly up-regulated the expression of CD11c, CD80, CD83, CD86, CD274 and MHC class II in whole-blood mDCs and CD80, CD123 and MHC class II in whole-blood pDCs. Down-regulation of CD195 expression in both DC subpopulations was also noted. Further analysis showed that HK M. obuense up-regulated the expression of CD80, CD83 and MHC class II on purified blood DC subpopulations. TLR2 and TLR1 were also identified to be engaged in mediating the HK M. obuense-induced up-regulation of surface receptor expression on whole blood mDCs. In addition, our data demonstrated that HK M. obuense augmented the secretion of CCL4, CCL5, CCL22, CXCL8, IL-6, IL-12p40 and TNF-α by purified total blood DCs. Taken together, our data suggest that HK M. obuense exerts potent differential immunomodulatory effects on human DC subpopulations.
URI: https://scholarhub.balamand.edu.lb/handle/uob/2097
DOI: 10.1177/1753425917727838
Ezproxy URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Biology

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