Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/2030
Title: Galactosyl- and fucosyltransferases in etiolated pea epicotyls : product identification and sub-cellular localisation
Authors: Baydoun, Elias A-H.
Abdel-Massih, Roula
Dani, Danielle
Rizk, Sandra E.
Brett, Cristopher T.
Affiliations: Department of Biology 
Keywords: Fucosyltransferase
Galactan
Galactosyltransferase
Glycoprotein
Pea epicoty
Xyloglucan
Subjects: Golgi apparatus
Issue Date: 2001
Part of: Journal of plant physiology
Volume: 158
Issue: 2
Start page: 145
End page: 150
Abstract: 
Particulate membrane preparations from etiolated pea epicotyls were found to contain fucosyltransferases, which transferred fucose from GDP-fucose onto xyloglucan and N-linked glycoprotein, and galactosyltransferases, which transferred galactose from UDP-galactose onto galactan, xyloglucan, and N-linked glycoprotein. The products were characterised by specific enzyme degradation and by acid and alkaline hydrolysis. All the enzymes were found to be concentrated in the Golgi apparatus. The Golgi apparatus was further fractionated into membranes of low, medium and high-density. The glycoprotein fucosyltransferase activity was present in highest amounts in the medium-density Golgi membranes, while the majority of the xyloglucan fucosyltransferase was present in the low-density Golgi membranes. The majority of the galactan galactosyltransferase (galactan synthase) was found in the low-density membranes, while the glycoprotein galactosyltransferase was equally distributed in all three subfractions.
URI: https://scholarhub.balamand.edu.lb/handle/uob/2030
DOI: 10.1078/0176-1617-00068
Ezproxy URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Biology

Show full item record

SCOPUSTM   
Citations

12
checked on Oct 23, 2021

Google ScholarTM

Check

Dimensions Altmetric

Dimensions Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.