Please use this identifier to cite or link to this item:
Title: Cytotoxic effect of Montivipera bornmuelleri's venom on cancer cell lines : in vitro and in vivo studies
Authors: Haddoub, Carol
Rima, Mohamad
Heurtebise, Sandrine
Lawand, Myriam
Jundi, Dania
Sadek, Riyad
Amigorena, Sebastian
Fajloun​, Ziad
Karam, Marc 
Affiliations: Department of Biology 
Keywords: Montivipera bornmuelleri
B16 cells
MCA cells
Anti-cancer activity
In vivo
Issue Date: 2020
Part of: Journal of peer j
Volume: 8
Background Montivipera bornmuelleris venom has shown immunomodulation of cytokines release in mice and selective cytotoxicity on cancer cells in a dose-dependent manner, highlighting an anticancer potential. Here, we extend these findings by elucidating the sensitivity of murine B16 skin melanoma and 3-MCA-induced murine fibrosarcoma cell lines to M. bornmuelleris venom and its effect on tumor growth in vivo. Methods The toxicity of the venom on B16 and MCA cells was assessed using flow cytometry and xCELLigence assays. For in vivo testing, tumor growth was followed in mice after intratumoral venom injection. Results The venom toxicity showed a dose-dependent cell death on both B16 and MCA cells. Interestingly, overexpression of ovalbumin increased the sensitivity of the cells to the venom. However, the venom was not able to eradicate induced-tumor growth when injected at 100 µg/kg. Our study demonstrates a cytotoxic effect of M. bornmuelleris venom in vitro which, however, does not translate to an anticancer action in vivo.
DOI: 10.7717/peerj.9909
Open URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Biology

Show full item record

Google ScholarTM


Dimensions Altmetric

Dimensions Altmetric

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.