Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/1591
Title: Additive effect of MCP in combination with cefotaxime against staphylococcus aureus
Authors: Dahdouh, Elias
EL Khatib, Salah
Baydoun, Elias A-H.
Abdel-Massih, Roula
Affiliations: Department of Biology 
Keywords: Antibiotic resistance
Checkerboard
Combination therapy
Modified Citrus Pectin
MRSA
Staphylococcus aureus
Issue Date: 2017
Part of: Medicinal chemistry
Volume: 13
Issue: 7
Start page: 682
End page: 688
Abstract: 
Background: Pectin is a heterogeneous polysaccharide present in plants and citrus fruits. It exhibits different beneficial biological activities. Conflicting reports exist about the antimicrobial effect of pectin and its derivatives. Objective: In this study, we investigate the antimicrobial effect of Modified Citrus Pectin (MCP) against Staphylococcus aureus, a pathogen showing increasing rates of antimicrobial resistance worldwide. Method: Forty-three clinical isolates of S. aureus were obtained from a hospital in North Lebanon. Minimum Inhibitory Concentrations (MICs) and Minimum Bactericidal Concentrations (MBCs) were determined using MCP after determining its optimum pH activity. The combination between MCP and cefotaxime was then investigated for S. aureus isolates using the checkerboard technique. Results and Discussion: The optimum pH for the activity of MCP was 6.0. MIC and MBC values against S. aureus ranged between 0.39-50 µg/µl and 3.13-50 µg/µl, respectively. These values are promising for using MCP in the inhibition of some S. aureus isolates at relatively low concentrations. Combination experiments showed an additive effect in most S. aureus strains between MCP and cefotaxime, and a synergistic effect in two strains. These preliminary findings open the way for further investigation into the therapeutic potential of MCP in the treatment of S. aureus infections. Conclusion: MCP demonstrates in vitro antimicrobial activity alone and in combination with cefotaxime against S. aureus. Keywords: Antibiotic resistance, checkerboard, combination therapy, modified citrus pectin, MRSA, Staphylococcus aureus.
URI: https://scholarhub.balamand.edu.lb/handle/uob/1591
Type: Journal Article
Appears in Collections:Department of Biology

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