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Title: The bioavailability of the Epidermal Growth Factor (EGF) in umbilical cord tissues as a perspective for stem-cell secretome-mediated skin rejuvenation mechanism
Authors: Fares, Reina
Advisors: Alaaeddine, Nada
Keywords: Umbilical cord, EGF, stem cells, skin regeneration, anti-aging treatment
Issue Date: 2023
The skin is a sensor element that displays the progress of time. Skin structure and
functions deteriorate because of biological and physical aging. Aging influences the skin
barrier, the elastic and mechanical characteristics of the epidermal cell membranes, as
well as its vascular reactivity. Barrier malfunction in old skin is caused by problems in
epidermal keratinocyte renewal and differentiation, which are specifically connected to
aberrant production of microRNAs that regulate cell division and senescence. The
mechanical characteristics of the dermis in old skin are altered by an aberrant balance of
matrix protein production and breakdown.
Skin-related problems have been rising recently and the role of stem cells in treatment
options attracted the interest of the research community. Several studies addressed the
potential of stem cells in wound healing and skin aging treatment, thus marking them as
agents of regenerative medicine. Stem cells of the umbilical cord were given a special
interest for their role and capability in fighting skin aging. Their role was linked to their
secreted paracrine factors including cytokines, chemokines, growth factors, and other
bioactive molecules. In this study, the bioavailability of the epidermal growth factor
(EGF) in the secretome derived from umbilical cord extracts (UC-explants) and
mesenchymal stem cells (UC-MSCs) is assessed using ELISA. EGF was found with a
maximum concentration of 22 pg/ml in secretome derived from UC-explants after
1week culture compared to the secretome derived from UC-MSCs which is the highest
in the first passage with a concentration of 4 pg/ml.
This anti-aging growth factor might contribute to skin rejuvenation and delay skin aging.
Includes bibliographical references (p. 39-66)
Rights: This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the personal and educational use exceptions must be obtained from the copyright holder
Ezproxy URL: Link to full text
Type: Thesis
Appears in Collections:UOB Theses and Projects

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