Please use this identifier to cite or link to this item:
Title: Mathematical modeling of ABE fermentation on glucose substrate with Zn supplementation for enhanced butanol production
Authors: Chalhoub, Elie
Nassar, Nathalie
Hawly, Mhamad
Belovich, Joanne M
Affiliations: Department of Chemical Engineering 
Department of Chemical Engineering 
Keywords: ABE fermentation
Mathematical model
Zinc supplement
Issue Date: 2023-10-01
Publisher: National Library of Medicine
Part of: Archives of Biochemistry and Biophysics
Volume: 747
Supplementation or limitation of some micronutrients during acetone-butanol-ethanol (ABE) fermentation has led to improvement in butanol yield and productivity. A mechanistic model of ABE fermentation offers insights in understanding these complex interactions and improving productivity through optimal culture conditions. This study proposes a mechanistic kinetic model of ABE fermentation by two Clostridium Acetobutylicum strains, L7 and ATCC 824 using glucose as sole carbon source without zinc and with various zinc doses. The model incorporates enzyme regulation by zinc on several glycolytic, acidogenesis and solventogenesis enzymes. The model was fitted and validated to experimental data collected from the published literature. The simulated results were in compliance with the experimental data, most importantly indicating higher glucose consumption and butanol productivity when supplemented with zinc compared to the control culture. The average squared correlation factor (R2) between the experimental and the simulated results, without and with zinc, were 0.99 and 0.96 for glucose, and 0.89 and 0.95 for butanol, respectively. A sensitivity analysis performed on the fitted and validated model indicated that the glucose consumption and growth parameters most influenced the model outputs. The developed model can be used as a template for modeling ABE fermentation under different combinations of micronutrients that may offer improved butanol yield and productivity.
ISSN: 00039861
DOI: 10.1016/
Ezproxy URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Chemical Engineering

Show full item record


checked on Apr 20, 2024

Record view(s)

checked on Apr 23, 2024

Google ScholarTM


Dimensions Altmetric

Dimensions Altmetric

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.