Effect of bee venom on hyperalgesia hot-plate latency

Abstract

Pain is a worldwide health problem that can affect the quality of life and result in psychological, physical, and social consequences. Hyperalgesia is an increased sensitivity to feeling pain and an extreme response to pain. Bee Venom (BV) from the honeybee can help in the treatment of hyperalgesia by reducing its nociceptive behavior. Formalin is the pain-inducing agent used in this research. The hot plate test is used to measure the hot plate latency of mice. In this study, the effect of bee venom on the hot-plate latency related to hyperalgesia and whether BV alone or BV combined with formalin provides the most efficient anti-nociceptive treatment for hyperalgesia were investigated. A 100 μl injection of BV was given to each mouse in the BV group and the formalin+BV group at a dose of 0.1 mg/kg. The formalin group and the formalin+BV group each received a subcutaneous injection of 50 μl of 5% formalin into the left hind paw 30 minutes following the BV injection. One-way ANOVA was carried out using Prism 9 software to compare the hot plate latencies between the four groups as a function of injection time and at each individual time point. The findings of this study indicated that BV might be an effective anti-nociceptive treatment for hyperalgesia. However, it has been demonstrated that formalin and BV operate more effectively together than BV does alone to produce a long-lasting anti-nociceptive effect.