Please use this identifier to cite or link to this item:
|Title:||Metformin Inhibits ROS Production by Human M2 Macrophages via the Activation of AMPK||Authors:||Nassif, Rana M
Dang, Pham My-Chan
|Affiliations:||Faculty of Health Sciences||Keywords:||AMPK
|Issue Date:||2022-01-29||Publisher:||National Library of Medicine||Part of:||Biomedicines||Volume:||10||Issue:||2||Abstract:||
Metformin (1,1-dimethylbiguanide hydrochloride) is the most commonly used drug to treat type II diabetic patients. It is believed that this drug has several other beneficial effects, such as anti-inflammatory and anticancer effects. Here, we wanted to evaluate the effect of metformin on the production of reactive oxygen species (ROS) by human macrophages. Macrophages are generated in vivo from circulating monocytes depending on the local tissue environment. In vitro proinflammatory macrophages (M1) and anti-inflammatory macrophages (M2) can be generated by culturing monocytes in the presence of different cytokines, such as GM-CSF or M-CSF, respectively. We show that metformin selectively inhibited human monocyte differentiation into proinflammatory macrophages (M1) without inhibiting their differentiation into anti-inflammatory macrophages (M2). Moreover, we demonstrate that, in response to LPS, M2 macrophages produced ROS, which could be very harmful for nearby tissues, and metformin inhibited this process. Interestingly, metformin with LPS induced activation of the adenosine-monophosphate-activated protein kinase (AMPK) and pharmacological activation of AMPK by AICAR, a known AMPK activator, decreased ROS production, whereas the deletion of AMPK in mice dramatically enhanced ROS production in different types of immune cells. These results suggest that metformin exhibits anti-inflammatory effects by inhibiting the differentiation of human monocytes into M1 macrophages and by limiting ROS production by macrophages via the activation of AMPK.
|URI:||https://scholarhub.balamand.edu.lb/handle/uob/5601||ISSN:||2227-9059||DOI:||10.3390/biomedicines10020319||Open URL:||Link to full text||Type:||Journal Article|
|Appears in Collections:||Department of Medical Laboratory Sciences|
Show full item record
checked on Jul 3, 2022
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.