Please use this identifier to cite or link to this item:
|Title:||The effect of experimental colitis on glucose absorption and aquaporin expression in the small intestine in adult rats||Other Titles:||The effect of experimental colitis on glucose absorption & aquaporin expression in the small intestine in adult rats||Authors:||Hwalla, Samih||Advisors:||Chakass, Dania Nadim.||Subjects:||Intestinal absorption
The aim of this research work is to study the effect of colitis or colonic inflammation induced by IA (iodoacetamide), TNBS (trinitrobenzene sulfonic acid) and DSS (dextransulfate sodium), on the absorptive capacity of the small intestine for glucose and water in adult Sprague-Dawley rats. The rate of glucose absorption was examined by the in vivo single pass perfusion technique in the jejunum and ileum and the mRNA level of expression of glucose transporters and aquaporins was determined in the small intestine in experimental colitis. The results have shown a significant decrease in the absorptive capacity for glucose in the jejunum, but not in the ileum. In addition, a significant decrease in the level of mRNA expression of SGLT1, GLUT2, GLUT5, AQP1, AQP7, AQP8 and Na+ -K+ -ATPase in the small intestine in all experimental colitis models. Moreover, a significant decrease of AQP1, AQP8 and Na+ -K+ -ATPase mRNA expression was detected in the colon. The present research indicated that a cross-talk exist between the inflamed colon and the small intestine. We suggest that this cross-talk may be controlled by a neural and/or an immunological mechanism.
Includes bibliographical references (p.72-78).
Supervised by Dr. Dania Awad.
|URI:||https://scholarhub.balamand.edu.lb/handle/uob/4246||Rights:||This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the personal and educational use exceptions must be obtained from the copyright holder||Ezproxy URL:||Link to full text||Type:||Thesis|
|Appears in Collections:||UOB Theses and Projects|
Show full item record
checked on Oct 15, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.