Please use this identifier to cite or link to this item:
Title: Ubiquinone is not required for proton conductance by uncoupling protein 1 in yeast mitochondria
Authors: Esteves, Telma C.
Echtay, Karim 
Jonassen, Tanya
Clarke, Catherine F.
Brand, Martin D
Affiliations: Faculty of Medicine 
Keywords: Proton conductance
Retinoic acid
Ubiquinone (coenzyme Q)
UCP1 (uncoupling protein 1)
Subjects: Mitochondria
Issue Date: 2004
Part of: Biochemical journal
Volume: 379
Issue: 2
Start page: 309
End page: 315
Q (coenzyme Q or ubiquinone) is reported to be a cofactor obligatory for proton transport by UCPs (uncoupling proteins) in liposomes [Echtay, Winkler and Klingenberg (2000) Nature (London) 408, 609–613] and for increasing the binding of the activator retinoic acid to UCP1 [Tomás, Ledesma and Rial (2002) FEBS Lett. 526, 63–65]. In the present study, yeast (Saccharomyces cerevisiae) mutant strains lacking Q and expressing UCP1 were used to determine whether Q was required for UCP function in mitochondria. Wild-type yeast strain and two mutant strains (CENΔCOQ3 and CENΔCOQ2), both not capable of synthesizing Q, were transformed with the mouse UCP1 gene. UCP1 activity was measured as fatty acid-dependent, GDP-sensitive proton conductance in mitochondria isolated from the cells. The activity of UCP1 was similar in both Q-containing and -deficient yeast mitochondria. We conclude that Q is neither an obligatory cofactor nor an activator of proton transport by UCP1 when it is expressed in yeast mitochondria.
Open URL: Link to full text
Type: Journal Article
Appears in Collections:Faculty of Medicine

Show full item record

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.