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|Title:||Ubiquinone is not required for proton conductance by uncoupling protein 1 in yeast mitochondria||Authors:||Esteves, Telma C.
Clarke, Catherine F.
Brand, Martin D
|Affiliations:||Faculty of Medicine||Keywords:||Proton conductance
Ubiquinone (coenzyme Q)
UCP1 (uncoupling protein 1)
|Subjects:||Mitochondria||Issue Date:||2004||Part of:||Biochemical journal||Volume:||379||Issue:||2||Start page:||309||End page:||315||Abstract:||
Q (coenzyme Q or ubiquinone) is reported to be a cofactor obligatory for proton transport by UCPs (uncoupling proteins) in liposomes [Echtay, Winkler and Klingenberg (2000) Nature (London) 408, 609–613] and for increasing the binding of the activator retinoic acid to UCP1 [Tomás, Ledesma and Rial (2002) FEBS Lett. 526, 63–65]. In the present study, yeast (Saccharomyces cerevisiae) mutant strains lacking Q and expressing UCP1 were used to determine whether Q was required for UCP function in mitochondria. Wild-type yeast strain and two mutant strains (CENΔCOQ3 and CENΔCOQ2), both not capable of synthesizing Q, were transformed with the mouse UCP1 gene. UCP1 activity was measured as fatty acid-dependent, GDP-sensitive proton conductance in mitochondria isolated from the cells. The activity of UCP1 was similar in both Q-containing and -deficient yeast mitochondria. We conclude that Q is neither an obligatory cofactor nor an activator of proton transport by UCP1 when it is expressed in yeast mitochondria.
|URI:||https://scholarhub.balamand.edu.lb/handle/uob/2678||Open URL:||Link to full text||Type:||Journal Article|
|Appears in Collections:||Faculty of Medicine|
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