Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/2629
Title: TAL-1/SCL and Its Partners E47 and LMO2 Up-Regulate VE-Cadherin Expression in Endothelial Cells
Authors: Deleuze, Virginie
Chalhoub, Elias
Hajj, Rawan El
Dohet, Christiane
Le Clech, Mikaël
Couraud, Pierre-Olivier
Huber, Philippe
Mathieu, Danièle
Affiliations: Department of Medical Laboratory Sciences 
Issue Date: 2007
Part of: Journal of molecular and cellular biology
Volume: 27
Issue: 7
Start page: 2687
End page: 2697
Abstract: 
The basic helix-loop-helix TAL-1/SCL essential for hematopoietic development is also required during vascular development for embryonic angiogenesis. We reported that TAL-1 acts positively on postnatal angiogenesis by stimulating endothelial morphogenesis. Here, we investigated the functional consequences of TAL-1 silencing in human primary endothelial cells. We found that TAL-1 knockdown caused the inhibition of in vitro tubulomorphogenesis, which was associated with a dramatic reduction in vascular endothelial cadherin (VE-cadherin) at intercellular junctions. Consistently, silencing of TAL-1 as well as of its cofactors E47 and LMO2 down-regulated VE-cadherin at both the mRNA and the protein level. Endogenous VE-cadherin transcription could be activated in nonendothelial HEK-293 cells by the sole concomitant ectopic expression of TAL-1, E47, and LMO2. Transient transfections in human primary endothelial cells derived from umbilical vein (HUVECs) demonstrated that VE-cadherin promoter activity was dependent on the integrity of a specialized E-box associated with a GATA motif and was maximal with the coexpression of the different components of the TAL-1 complex. Finally, chromatin immunoprecipitation assays showed that TAL-1 and its cofactors occupied the VE-cadherin promoter in HUVECs. Together, these data identify VE-cadherin as a bona fide target gene of the TAL-1 complex in the endothelial lineage, providing a first clue to TAL-1 function in angiogenesis.
URI: https://scholarhub.balamand.edu.lb/handle/uob/2629
Type: Journal Article
Appears in Collections:Department of Medical Laboratory Sciences

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