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Title: Lung endothelial barrier disruption in Lyl1-deficient mice
Authors: Pirot, Nelly
Delpech, Hélène
Deleuze, Virginie
Dohet, Christiane
Courtade-Saïdi, Monique
Basset-Léobon, Céline
Chalhoub, Elias
Mathieu, Danièle
Pinet, Valérie
Affiliations: Department of Medical Laboratory Sciences 
Keywords: lymphoblastic leukemia-derived sequence 1
Vascular endothelial-cadherin
Vascular permeability
Issue Date: 2014
Part of: American journal of physiology-lung cellular and molecular physiology
Volume: 306
Issue: 8
Start page: 775
End page: 785
Maturation of newly formed vessels is a multistep phenomenon during which functional endothelial barriers are established. Disruption of vessel integrity is an important feature in many physiological and pathological processes. We previously reported that lymphoblastic leukemia-derived sequence 1 (LYL1) is required for the late stages of postnatal angiogenesis to limit the formation of new blood vessels, notably by regulating the activity of the small GTPase Rap1. In this study, we show that LYL1 is also required during the formation of the mature endothelial barrier in the lungs of adult mice. Specifically, LYL1 knockdown in human endothelial cells downregulated the expression of ARHGAP21 and ARHGAP24, which encode two Rho GTPase-activating proteins, and this was correlated with increased RhoA activity and reorganization of the actin cytoskeleton into stress fibers. Importantly, in lungs of Lyl1-deficient mice, both vascular endothelial (VE)-cadherin and p120-catenin were poorly recruited to endothelial adherens junctions, indicative of defective cell-cell junctions. Consistent with this, higher Evans blue dye extravasation, edema, and leukocyte infiltration in the lung parenchyma of Lyl1−/− mice than in wild-type littermates confirmed that lung vascular permeability is constitutively elevated in Lyl1−/− adult mice. Our data show that LYL1 acts as a stabilizing signal for adherens junction formation by operating upstream of VE-cadherin and of the two GTPases Rap1 and RhoA. As increased vascular permeability is a key feature and a major mechanism of acute respiratory distress syndrome, molecules that regulate LYL1 activity could represent additional tools to modify the endothelial barrier permeability. The mature continuous vascular endothelium that lines blood vessels forms a selective and semipermeable barrier to control the exchange of solutes, proteins, fluids, and immune cells between blood and tissues. Fine regulation of endothelial permeability is required i.
DOI: 10.1152/ajplung.00200.2013
Open URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Medical Laboratory Sciences

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