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Title: Inactivation of plasmodium falciparum in whole blood by riboflavin plus irradiation
Authors: Chaar, Mira El 
Freimanis, Graham L.
Allain, Jean-Pierre
Atwal, Sharan
Dinko, Bismarck
Affiliations: Department of Medical Laboratory Sciences 
Issue Date: 2013
Part of: Journal of transfusion
Volume: 53
Issue: 12
Start page: 3174
End page: 3183
Background Malaria parasites are frequently transmitted by unscreened blood transfusions in Africa. Pathogen reduction methods in whole blood would thus greatly improve blood safety. We aimed to determine the efficacy of riboflavin plus irradiation for treatment of whole blood infected with Plasmodium falciparum. Study Design and Methods Blood was inoculated with 104 or 105 parasites/mL and riboflavin treated with or without ultraviolet (UV) irradiation (40-160 J/mL red blood cells [mLRBCs]). Parasite genome integrity was assessed by quantitative amplification inhibition assays, and P. falciparum viability was monitored in vitro. Results Riboflavin alone did not affect parasite genome integrity or parasite viability. Application of UV after riboflavin treatment disrupted parasite genome integrity, reducing polymerase-dependent amplification by up to 2 logs (99%). At 80 J/mLRBCs, riboflavin plus irradiation prevented recovery of viable parasites in vitro for 2 weeks, whereas untreated controls typically recovered to approximately 2% parasitemia after 4 days of in vitro culture. Exposure of blood to 160 J/mLRBCs was not associated with significant hemolysis. Conclusions Riboflavin plus irradiation treatment of whole blood damages parasite genomes and drastically reduces P. falciparum viability in vitro. In the absence of suitable malaria screening assays, parasite inactivation should be investigated for prevention of transfusion-transmitted malaria in highly endemic areas.
DOI: 10.1111/trf.12235
Ezproxy URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Medical Laboratory Sciences

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