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Title: Combining moderate pulsed electric fields with temperature and with organic acids to inactivate Escherichia coli suspensions
Authors: Zakhem, Henri El 
Lanoisellé, Jean-Louis
Lebovka, Nikolai I.
Nonus, Maurice
Vorobiev, Eugene
Affiliations: Department of Chemical Engineering 
Keywords: Escherichia coli
Pulsed electric field
Thermal treatment
Electrical conductivity
Organic acids
Issue Date: 2007
Part of: IIUM engineering journal
Volume: 7
Issue: 2
Start page: 1
End page: 8
The aim of this work was to study the efficiency of inactivation of Escherichia coli cells in aqueous suspensions using combined moderate pulsed electric field (PEF) and thermal treatments. The inactivation kinetics of E. coli cells in aqueous suspensions (1 wt%) was monitored using conductometric technique. The electric field strength E was within 5-7.5 kV/cm, the effective PEF treatment time tPEF was within 0-0.75 s, the pulse duration ti was within 0.3-1 ms, the medium temperature was 30-50°C, and the time of thermal treatment tT was within 0-7000 s. The organic acid concentration was within 0-0.5 g/L.The damage of E. coli was accompanied by release of intracellular components. The synergy between the PEF and thermal treatments in E. coli inactivation was clearly demonstrated. The damage efficiency was noticeably improved by addition of organic acids, especially lactic acid.
Open URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Chemical Engineering

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