A comparative in vitro study of the effect of secretome derived from umbilical cord mesenchymal stem cells versus cisplatin chemotherapy on ovarian cancer cell line

Abstract

Ovarian cancer is a highly malignant gynecological cancer that mostly originates from surface epithelium of ovaries. A major drawback is resistance and recurrence to standard treatment, such as Cisplatin. Secretome, known as (conditioned media), derived from mesenchymal stem cells, is a set of factors secreted into the extracellular space and is proposed as a novel anti-tumor agent. Umbilical cord mesenchymal stem cells derived secretome have demonstrated both pro-and anti-tumor effect. In this research, a series of assays will be employed to explore the effect of umbilical cord derived secretome on SKOV3 ovarian cancer cell line compared to cisplatin. MTT assay to assess proliferation, ELISA for tumor markers (CA-125 and β-hCG) and, RT-qPCR for the expression of IL-6, IL-8, IL-10 and MMP-9. Our findings demonstrated discrepancy in the results, secretome decreased proliferation, IL 6, IL-10, CA-125, β-hCG expression and increased MMP-9 and IL-8 expression compared to cisplatin. More scientific investigations are needed to propose secretome as a sensitization treatment against ovarian cancer