Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/7594
Title: Molecular epidemiology of vancomycin resistant enterococcus in tertiary care hospitals in Lebanon
Authors: Mroue, Maya
Advisors: Hajjar, Micheline
Keywords: Vancomycin resistant Enterococci, antimicrobial resistance, vanA, vanB polymerase chain reaction.
Subjects: University of Balamand--Dissertations
Dissertations, Academic
Issue Date: 2024
Publisher: [Kalhat, Lebanon] : [University of Balamand], 2024
Abstract: 
Background: The occurrence of infections caused by vancomycin-resistant Enterococcus
(VRE) is on the rise, accompanied by high mortality rates and restricted treatment options.
Various gene clusters contribute to vancomycin resistance in Enterococcus, with the vanA
and vanB genes being the most clinically significant. There is scarce data on the molecular
characterization of VRE in Lebanon.
Objectives: This study aimed to conduct a genotypic analysis of VRE isolates collected from
clinical samples in two hospitals in Lebanon. Clinical characteristics associated with VRE
infections were also investigated.
Methods: Bacterial isolates were from patients with VRE infections admitted to Saint George
Hospital University Medical Centre (SGHUMC) and Hammoud Hospital University Medical
Centre (HHUMC) between 2019 to 2020 and 2023 to 2024 respectively along with
corresponding clinical data. Vancomycin resistance genes, including vanA and vanB, were
amplified and identified in 29 VRE isolates using polymerase chain reaction (PCR).
Results: All 29 (100%) VRE isolates carried the vanA gene, whereas none of the isolates
carried vanB. Blood specimens yielded the highest percentage (48%), followed by urine
(34.5%) of isolates from which VRE was obtained, with most patients affected being aged
above 60 years.
Conclusion: This study confirms that VRE isolates harbouring vanA genes are prevalent in
the area of the study. PCR is a highly reliable, sensitive and specific method that enables the
rapid detection of vancomycin resistance genes.
Description: 
Includes bibliographical references (p. 39-50)
URI: https://scholarhub.balamand.edu.lb/handle/uob/7594
Rights: This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the personal and educational use exceptions must be obtained from the copyright holder
Ezproxy URL: Link to full text
Type: Thesis
Appears in Collections:UOB Theses and Projects

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