Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/7299
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dc.contributor.authorEl Tannir, Hanaen_US
dc.contributor.authorHouhou, Dianaen_US
dc.contributor.authorDebs, Esperanceen_US
dc.contributor.authorKoubaa, Mohameden_US
dc.contributor.authorJammoul, Adlaen_US
dc.contributor.authorAzakir, Bilalen_US
dc.contributor.authorKhalil, Mahmoud Ien_US
dc.contributor.authorEl Darra, Nadaen_US
dc.contributor.authorLouka, Nicolasen_US
dc.contributor.editorProf. Dr. Massimo Negrinien_US
dc.date.accessioned2024-04-03T06:22:07Z-
dc.date.available2024-04-03T06:22:07Z-
dc.date.issued2024-03-21-
dc.identifier.urihttps://scholarhub.balamand.edu.lb/handle/uob/7299-
dc.description.abstractBackground: Cumin seeds, extracted from the plant Cuminum cyminum, are abundant in phenolic compounds and have been extensively researched for their chemical makeup and biological effects. The objective of this research is to enhance the water extraction of polyphenols through the water bath (WB) technique and to evaluate the antiradical, antibacterial, and anticancer effects of the extract. (2) Methods: Response Surface Methodology was used to find the best parameters to extract polyphenols. Three experimental parameters, time, temperature, and solid-liquid ratio, were tested. The disc diffusion method has been used to determine the antimicrobial activities against Salmonella Typhimurium, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Candida albicans. The antiradical activity was performed using the DPPH method, while total phenolic content was performed using Folin–Ciocalteu. High-Performance Liquid Chromatography (HPLC) was conducted to analyze the phytochemical profile of WB extracts. The anticancer activity of the lyophilized extract was assessed against three cancer cell lines (colon (HT29), lung (A549), and breast (MCF7) cancer cell lines).; (3) Results: The optimal conditions for water extraction were 130 min at 72 °C. The total phenolic compounds yield (14.7 mg GAE/g DM) and antioxidant activity (0.52 mg trolox eq./mL) were obtained using a 1:40 solid–liquid ratio. The primary polyphenols identified were the flavonoids rutin (0.1 ppm) and ellagic acid (3.78 ppm). The extract had no antibacterial or antifungal activities against the microorganisms tested. The extract showed anticancer activity of about 98% against MCF7 (breast cancer cell line), about 81% against HT29 (colon cancer cell line), and 85% against A549 (lung cancer cell line) at high doses. (4) Conclusions: Extraction time and a high solid–liquid ratio had a positive impact on polyphenol recovery and in maintaining their quantity and quality. Furthermore, the optimal aqueous extract exhibited strong antiradical activity reflected by the inhibition of free radicals in addition to a significant specificity against the tested cancer cell lines.en_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.subjectCuminum cyminumen_US
dc.subjectAnticancer activityen_US
dc.subjectAntimicrobial activityen_US
dc.subjectExtraction optimizationen_US
dc.subjectPolyphenolsen_US
dc.subjectResponse surface methodologyen_US
dc.titleOptimization of Aqueous Extraction of Polyphenols from Cuminum cyminum Seeds Using Response Surface Methodology and Assessment of Biological Activityen_US
dc.typeJournal Articleen_US
dc.identifier.doi10.3390/biotech13010007-
dc.identifier.pmid38534916-
dc.identifier.scopus2-s2.0-85188828350-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85188828350-
dc.contributor.affiliationDepartment of Biological Sciences, Faculty of Science, Beirut Arab University, Beirut P.O. Box 11-5020, Lebanonen_US
dc.contributor.affiliationDepartment of Nutrition and Dietetics, Faculty of Health Sciences, Beirut Arab University, Beirut P.O. Box 11-5020, Lebanonen_US
dc.contributor.affiliationDepartment of Biologyen_US
dc.contributor.affiliationUniversité de Technologie de Compiègne, ESCOM, TIMR (Integrated Transformations of Renewable Matter), Centre de Recherche Royallieu, CS 60319, CEDEX, 60203 Compiègne, Franceen_US
dc.contributor.affiliationLebanese Agricultural Research Institute, Food Department, Beirut P.O. Box 2611, Lebanonen_US
dc.contributor.affiliationMolecular and Translational Medicine Laboratory, Faculty of Medicine, Beirut Arab University, Beirut P.O. Box 11-5020, Lebanonen_US
dc.contributor.affiliationMolecular Biology Unit, Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21568, Egypten_US
dc.contributor.affiliationDepartment of Nutrition and Dietetics, Faculty of Health Sciences, Beirut Arab University, Beirut P.O. Box 11-5020, Lebanonen_US
dc.contributor.affiliationCentre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-Alimentaire, Faculté des Sciences, Saint-Joseph University, Beirut P.O. Box 17-5208, Lebanonen_US
dc.description.volume13en_US
dc.description.issue7en_US
dc.description.startpage1en_US
dc.description.endpage14en_US
dc.description.statusPublisheden_US
dc.identifier.openURLhttps://www.mdpi.com/2673-6284/13/1/7en_US
dc.relation.ispartoftextBiotechen_US
dc.rights.accessrightsOpen Accessen_US
crisitem.author.parentorgFaculty of Arts and Sciences-
Appears in Collections:Department of Biology
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