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Title: Colonisation [sic] colonization of hospitalized patients with carbapenem, 3GC resistant gram-negative organism : a longitudinal study
Authors: Khoury, Yara-Lynne El
Advisors: Chaar, Mira El 
Keywords: Epidemiology, E. coli, colonization, Longitudinal study, Whole-Genome Sequencing, ST131.
Issue Date: 2023
Background: Colonization with multidrug resistant E. coli strains causes a substantial health burden in hospitalized patients. We performed a longitudinal genomics study to investigate the colonization of resistant E. coli strains in critically ill patients, and to identify evolutionary changes and strain replacement events within patients. Methods: Patients were admitted to the intensive care unit and hematology wards at a major hospital in Lebanon. Perianal swabs were collected from participants on admission and during hospitalization, which were screened for extended-spectrum beta-lactamases and carbapenem-resistant Enterobacteria. We performed whole-genome sequencing and analysis on E. coli strains isolated from patients at multiple time points. Results: The E. coli isolates were genetically diverse, with 13 sequence types (STs) identified among 22 isolates sequenced. Five patients were colonized by E. coli sequence type ST 131 encoding CTX-M-27, an emerging clone not previously observed in clinical samples from Lebanon. Among the eight patients whose resident E. coli strains were tracked over time, five harbored the same E. coli strain with relatively few mutations over the 5 to 10 days of hospitalization. The other three patients were colonized by different E. coli strains over time. Conclusion: Our study provides evidence of strain diversity within patients during their hospitalization. While strains varied in their antimicrobial resistance profiles, the number of resistance genes did not increase over time. We also show that ST131 encoding CTX-M-27, which appears to be emerging as a globally important multidrug resistant E. coli strain, is also prevalent among critical care patients and deserves further monitoring.
Includes bibliographical references (p. 59-67)
Rights: This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the personal and educational use exceptions must be obtained from the copyright holder
Ezproxy URL: Link to full text
Type: Project
Appears in Collections:UOB Theses and Projects

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