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Title: Exploring the World of Membrane Proteins: Techniques and Methods for Understanding Structure, Function, and Dynamics
Authors: Boulos, Imad
Jabbour, Joy
Khoury, Serena
Mikhael, Nehme
Tishkova, Victoria
Candoni, Nadine
Ghadieh, Hilda 
Veesler, Stéphane
Bassim, Youssef 
Azar, Sami 
Harb, Frederic 
Affiliations: Faculty of Medicine 
Faculty of Medicine 
Faculty of Medicine 
Faculty of Medicine 
Keywords: X-ray crystallography
Artificial intelligence
Atomic force microscopy
Computational techniques
Cryo-electron microscopy
Membrane proteins
Nuclear magnetic resonance
Issue Date: 2023-10-19
Publisher: National Library of Medicine
Part of: Molecules (Basel, Switzerland)
Volume: 28
Issue: 20
In eukaryotic cells, membrane proteins play a crucial role. They fall into three categories: intrinsic proteins, extrinsic proteins, and proteins that are essential to the human genome (30% of which is devoted to encoding them). Hydrophobic interactions inside the membrane serve to stabilize integral proteins, which span the lipid bilayer. This review investigates a number of computational and experimental methods used to study membrane proteins. It encompasses a variety of technologies, including electrophoresis, X-ray crystallography, cryogenic electron microscopy (cryo-EM), nuclear magnetic resonance spectroscopy (NMR), biophysical methods, computational methods, and artificial intelligence. The link between structure and function of membrane proteins has been better understood thanks to these approaches, which also hold great promise for future study in the field. The significance of fusing artificial intelligence with experimental data to improve our comprehension of membrane protein biology is also covered in this paper. This effort aims to shed light on the complexity of membrane protein biology by investigating a variety of experimental and computational methods. Overall, the goal of this review is to emphasize how crucial it is to understand the functions of membrane proteins in eukaryotic cells. It gives a general review of the numerous methods used to look into these crucial elements and highlights the demand for multidisciplinary approaches to advance our understanding.
DOI: 10.3390/molecules28207176
Open URL: Link to full text
Type: Journal Article
Appears in Collections:Faculty of Medicine

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