Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/5660
Title: Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon
Authors: Nawfal Dagher, Tania
Al-Bayssari, Charbel
Chabou, Selma
Antar, Nadine
Diene, Seydina M
Azar, Eid
Rolain, Jean-Marc
Affiliations: Faculty of Medicine 
Keywords: Acinetobacter baumannii
OXA-23
ST2 clone
Issue Date: 2019
Publisher: Springer
Part of: BMC Microbiology
Volume: 19
Issue: 1
Abstract: 
Background
Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-resistant A. baumannii isolates collected from Saint-Georges Hospital in Lebanon.

Methods
Between January and August 2016, 31 A. baumannii isolates were collected from sputum samples of patients infected with ventilator-associated pneumonia (VAP) and treated with colistin-carbapenem combination therapy. Antibiotic susceptibility testing was performed using the disk diffusion method. Carbapenemases, extended spectrum β-lactamases encoding genes and mcr-1/2 genes were investigated by RT-PCR and standard PCR. The epidemiological relatedness of the strains was studied using MLST analysis.

Results
Most of the isolates exhibited multidrug-resistant phenotypes. All the isolates were carbapenem-resistant and among them, 30 carried the class D carbapenemase blaoxa-23 gene while one isolate carried blaoxa-72 gene. MLST results revealed three sequence types, namely ST2, ST699, and ST627. Isolates having ST2 were the most prevalent clone (29/31, 93.5%).

Conclusions
This study shows a nosocomial spread of multidrug-resistant A. baumannii ST2 having blaOXA-23 gene in Saint-George in Lebanon. Monitoring and control measures need to be adopted to avoid the spread of A. baumannii to patients.
URI: https://scholarhub.balamand.edu.lb/handle/uob/5660
DOI: 10.1186/s12866-019-1401-2
Open URL: Link to full text
Type: Journal Article
Appears in Collections:Faculty of Medicine

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