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Title: Magnolia officinalis ameliorates dehydroepiandrosterone-induced polycystic ovary syndrome in rats
Authors: Nassar, Aline
Khachab, Maha 
Zaatiti, Hayat 
Kanaan, Amjad 
Affiliations: Faculty of Medicine 
Faculty of Medicine 
Faculty of Medicine 
Keywords: Insulin Resistance
Magnolia officinalis
Polycystic ovary syndrome
Issue Date: 2021-01-01
Part of: Jundishapur Journal of Natural Pharmaceutical Products
Volume: 16
Issue: 3
Background: Polycystic ovary syndrome (PCOS) is a prevalent reproductive and metabolic disorder. Insulin resistance (IR) is highly associated with PCOS and aggravates its symptoms. Thiazolidinediones (TZDs), as insulin sensitizing agents, are PPAR agonists that improve many of the symptoms of PCOS. The Magnolia officinalis extract (MOE) is a natural peroxisome proliferator activated receptor gamma (PPAR) agonist that improves insulin sensitivity in experimental models. Objectives: Using a dehydroepiandrosterone (DHEA)-induced rat model of PCOS and IR, this study aimed to explore both the potential beneficial effects and the molecular mechanisms of action of MOE. Methods: Post-pubertal female Sprague Dawley rats were subcutaneously injected daily with DHEA (6 mg/100 g body weight) dissolved in sesame oil for 28 days (n = 30). Age-and weight-matched control rats received only sesame oil (n = 12). Afterward, 16 of the DHEA-injected rats, along with five control rats, were sacrificed for blood and tissue collection. The 14 remaining DHEA-injected rats received either treatment of 30 days of oral MOE (500 mg/kg) dissolved in dimethyl sulfoxide (DMSO) (n = 7), or oral DMSO only (n = 7). Meanwhile, the remaining control rats (n = 7) continued to receive daily oral DMSO for 30 days. At the end of the treatments, the rats were sacrificed for blood and tissue collection. Results: After 28 days, the DHEA-Treated rats exhibited an increase in body weight as compared to controls (P 0.05). DHEA injection induced a PCOS phenotype as evident by a statistically significant (P 0.05) elevated serum luteinizing hormone (LH), and an increased number of cystically dilated follicles with thicker granulosa compared to controls. PCOS rats showed a statistically significant rise in fasting insulin with an increased homeostatic model assessment index of insulin resistance (HOMA-IR) as compared to controls (P 0.05). Compared to the control group, PCOS rats had a statistically significant lower ovarian protein expression of PPAR, insulin receptor substrate 1 (IRS1), and protein kinase B (Akt) byWestern Blot (P 0.05). Conversely, the PCOS group showed an increased mammalian target of rapamycin (mTOR) pathway activity as evident by an increase in the fraction of phosphorylated mTOR to total mTOR compared to the control group (P 0.05). When treated for 30 days with oral MOE (500 mg/kg), the PCOS rats showed a statistically significant decrease in body weight and serum LH levels as compared to the non-Treated PCOS rats (P 0.05). The number of cystically dilated follicles in the MOE-Treated PCOS rats was significantly reduced compared to the non-Treated PCOS rats. In the MOE-Treated PCOS rats, the ovarian protein expression of PPAR, IRS1, and Akt was significantly increased, while the p-mTOR/mTOR expression was decreased compared to the non-Treated PCOS group (P 0.05). Conclusions: According to our results, the MOE ameliorated the DHEA-induced PCOS phenotype histologically, hormonally, and metabolically. Fundamentally, this explores the elusive pathophysiologic association between IR and PCOS by targeting pathways common to both disorders.
ISSN: 17357780
DOI: 10.5812/JJNPP.106447
Open URL: Link to full text
Type: Journal Article
Appears in Collections:Faculty of Medicine

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