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Title: The effect of total colectomy on water absorption and aquaporin expression in the rat jejunum
Other Titles: The effect of total colectomy on water absorption & aquaporin expression in the rat jejunum
Authors: Osman, Samira
Advisors: Khachab, Maha 
Subjects: Gastrointestinal system--Physiology
Issue Date: 2013
The main role of the colon is electrolyte and water absorption. Total colectomy eliminates this colonic function and may alter the absorptive capacity if the small intestine for nutrients and fluid. The purpose of the present study was to investigate the effect of total colectomy on water absorption in the jejunum in colectomized rats that were allowed to recover for 3 days, 15 days and 30 days post-colectomy. mRNA and protein expression of the genes coding for water transporters AQP1, AQP4, AQP7 and AQP8 were analyzed by RT-PCR and Western Blot. The results have revealed no significant changed in mRNA expression of aquaporins whereas a significant decrease in protein expression of AQP1, 4, 7 and 8 was seen at 15 days post-colectomy when compared to normal/sham rats before returning to normal at 30 days post-colectomy. However, no significant change in water absorption was detected in the jejunum post-colectomy when compared to normal/sham rats. These results indicate that a functional adaptation occurs in the small intestine following total colectomy, through the ability of the small intestine to modulate its absorptive capacity for water absorption following the loss of the colon.
Includes bibliographical references (p.51-55).

Supervised by Dr. Maha Khachab.
Rights: This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the personal and educational use exceptions must be obtained from the copyright holder
Ezproxy URL: Link to full text
Type: Thesis
Appears in Collections:UOB Theses and Projects

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