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Title: The mechanism of phenylbutyric acid-induced glucose uptake in 3T3-L1 adipocytes
Authors: Dahdah, Norma
Advisors: Kanaan, Amjad 
Subjects: Butyric acid
Diabetes Mellitus--Drug therapy
Issue Date: 2017
Diabetes Mellitus is a chronic metabolic disease characterized by impaired glucose uptake and hyperglycemia. The impaired glucose uptake in adipocytes results from defects in insulin secretion and/or action. Phenylbutyric Acid (PBA), a short chain fatty acid, was found to increase glucose uptake in differentiated 3T3-L1 cells. The purpose of this study is to investigate the mechanism of this PBA-induced glucose uptake. Our findings suggest that PBA neither alters the viability nor the reactive oxygen species production of differentiated 3T3-L1 cells. PBA increases the plasma membrane abundance of the glucose transporter GLUT4 without a significant increase in its total protein expression. Alternatively, the total GLUT1 protein expression increase significantly in PBA-treated adipocytes compared to control. PBA reduces the activity of PTEN (a PI3-kinase pathway inhibitor) through phosphorylation of PTEN. Concomitantly, there is a significant increase in PIP3 and activation of Akt, as evident by its phosphorylation in the PBA-treated adipocytes. Our data suggest that PBA induces glucose uptake in 3T3-L1 adipocytes through affecting the expression of glucose transporters and/or their trafficking possibly via the PI3-kinase pathway. This offers PBA, or its modulators, as potential prospect targets for the management of Diabetes Mellitus.
Includes bibliographical references (p. 46-51).

Supervised by Dr. Amjad Kanaan.
Rights: This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the personal and educational use exceptions must be obtained from the copyright holder
Ezproxy URL: Link to full text
Type: Thesis
Appears in Collections:UOB Theses and Projects

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