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dc.contributor.advisorKaram, Marcen_US
dc.contributor.authorKanaan, Michelen_US
dc.descriptionIncludes bibliographical references (p.58-79).en_US
dc.descriptionSupervised by Dr. Marc Karam.en_US
dc.description.abstractCutaneous leishmaniasis is considered as a major endemic disease of public health. Organic antimony compounds known to be the most efficient treatment currently available are associated with cardiac toxicity and require careful monitoring. Thus, an inexpensive and safe systemic drug for Leishmania is urgently needed. Gentian Violet which is a triphenylmethane derivative, showed a remarkable in vitro and in vivo efficacy against parasites causing cutaneous leishmaniasis. In our research, we aim to investigate the efficiency of three different doses of Gentian Violet (1.25, 2.5 and 5 mg/kg) given through IP injections to L.major infected BALB/c mice. Most importantly, the effect of Gentian Violet on L.major-induced hyperalgesia was tested. In addition, in order to evaluate the Gentian Violet effect on immune responses, the levels of several cytokines (IFNγ, TNFα, IL-4, IL-10 and IL-17) were quantified. Interestingly, 2.5 mg/kg of Gentian Violet had no considerable effect on hyperalgesia and was the most efficient dose in reversing L.major-induced hyperalgesia. On the other hand, IL-4 and IL-10 levels were significantly decreased. These data suggest that Th2 response which normally leads to a susceptibility scenario was impaired by Gentian Violet treatment. In contrast, Th1 response which leads to resistance against the disease was not affected since IFNγ secretion was not modified. Furthermore, TNFα and IL-17 levels were not significantly affected.en_US
dc.description.statementofresponsibilityby Michel Kanaanen_US
dc.format.extentxi, 79 p. :ill. ;30 cmen_US
dc.rightsThis object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the personal and educational use exceptions must be obtained from the copyright holderen_US
dc.subject.lcshGentian Violeten_US
dc.titleThe Effect of Gentian Violet on Leishmania Major induced hyperalgesia and course of infection in BALB/C miceen_US
dc.title.alternativeThe effect of Gentian violet on leishmania major induced hyperalgesia & course of infection in BALB/c miceen_US
dc.contributor.departmentDepartment of Biologyen_US
dc.contributor.facultyFaculty of Arts and Sciencesen_US
dc.contributor.institutionUniversity of Balamanden_US
dc.description.degreeMSc in Biologyen_US
Appears in Collections:UOB Theses and Projects
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