Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/1921
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dc.contributor.authorAbdel-Massih, Roulaen_US
dc.contributor.authorBaydoun, Elias A-H.en_US
dc.contributor.authorWaldron, Keithen_US
dc.contributor.authorBrett, Cristopher T.en_US
dc.date.accessioned2020-12-23T09:02:54Z-
dc.date.available2020-12-23T09:02:54Z-
dc.date.issued2007-
dc.identifier.urihttps://scholarhub.balamand.edu.lb/handle/uob/1921-
dc.description.abstractPectins were extracted from roots and petioles of sugar beet, and treated with α-arabinosidase, 1,4-β-galactanase or polygalacturonase. They were then cross-linked using hydrogen peroxide and peroxidase. The effects on pectin molecular size were monitored by size-exclusion chromatography and viscometry. A decrease in apparent molecular size was observed after α-arabinosidase and polygalacturonase treatment, and all three enzymes caused a decrease in viscosity. The pectins were then cross-linked using hydrogen peroxide and peroxidase, and the effects on dehydrodiferulate formation were monitored by HPLC. Pretreatment with polygalacturonase caused no significant change in subsequent dehydrodiferulate cross-linking, while pretreatment with α-arabinosidase caused a slight change in the ratios of the different dehydrodiferulates formed. Pretreatment with 1,4-β-d-galactanase caused a more significant change in the ratios of the different dehydrodiferulates formed, and also greatly increased the overall recovery of total ferulates (monomers plus dehydrodiferulates), both in root pectin and petiole pectin. The possible effects of polysaccharide microstructure on the dimerisation and further polymerisation of pectin-linked ferulates are discussed.en_US
dc.format.extent5 p.en_US
dc.language.isoengen_US
dc.subjectBeta vulgaris Len_US
dc.subjectChenopodaciaceaeen_US
dc.subjectSugar beeten_US
dc.subjectCross-linkingen_US
dc.subjectFerulateen_US
dc.subjectDehydrodiferulateen_US
dc.subjectα-Arabinosidaseen_US
dc.subject1,4-β-Galactanaseen_US
dc.subjectPolygalacturonaseen_US
dc.subject.lcshPectinen_US
dc.titleEffects of partial enzymic degradation of sugar beet pectin on oxidative coupling of pectin-linked ferulates in vitroen_US
dc.typeJournal Articleen_US
dc.identifier.doi10.1016/j.phytochem.2007.04.007-
dc.contributor.affiliationDepartment of Biologyen_US
dc.description.volume68en_US
dc.description.issue13en_US
dc.description.startpage1785en_US
dc.description.endpage1790en_US
dc.date.catalogued2017-10-31-
dc.description.statusPublisheden_US
dc.identifier.ezproxyURLhttp://ezsecureaccess.balamand.edu.lb/login?url=https://doi.org/10.1016/j.phytochem.2007.04.007en_US
dc.identifier.OlibID174648-
dc.relation.ispartoftextPhytochemistryen_US
dc.provenance.recordsourceOliben_US
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