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Title: Competition assays between ESBL-producing E. coli and K. pneumoniae isolates collected from Lebanese elderly: an additional cost on fitness
Authors: Hafza, Nourhane
Challita, Caren
Dandachi, Imad
Dahdouh, Elias
Daoud, Ziad
Affiliations: Faculty of Medicine 
Keywords: MDROs
Fecal carriage
Fitness cost
Nursing homes
Subjects: Enterobacteriaceae
Issue Date: 2018
Part of: Journal of infection and public health
Volume: 11
Issue: 3
Start page: 393
End page: 397
The dissemination of Multi Drug Resistant Organisms (MDROs) is one of the major public health problems addressed nowadays. High fecal carriage rates of MDR Enterobacteriaceae were reported from Lebanese nursing homes. Studies have shown that the acquisition of resistance genes by bacteria might confer a fitness cost detected as a decrease in the frequency of these bacteria as compared to sensitive isolates. In this study, the competitive growth of MDR Enterobacteriaceae isolated from elderly is assessed. Sensitive and ESBL-producing Escherichia coli and Klebsiella pneumoniae isolates were identified. Inter-species in-vitro competition assays were conducted in different combinations. ESBL-producing K. pneumoniae presented a fitness cost when competing against sensitive E. coli. On the other hand, resistant E. coli only showed a fitness cost when growing in presence of two sensitive K. pneumoniae isolates. These results suggest that ESBL-production genes in E. coli and K. pneumoniae may confer a fitness cost that leads to the decrease in frequency of these bacteria in interspecies competitions. Culturing bacteria in a medium with more diverse isolates can provide better insights into bacterial competition and resistance dynamics, which can be exploited in the search for alternative therapeutic approaches towards the colonization of resistant bacteria.
DOI: 10.1016/j.jiph.2017.09.010
Ezproxy URL: Link to full text
Type: Journal Article
Appears in Collections:Faculty of Medicine

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