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Title: Colectomy induces an aldosterone-mediated increase in jejunal glucose uptake in rats
Authors: Khachab, Maha 
Kanaan, Amjad 
Dania, Awad
Deeba, Elie
Osman, Samira
Nassar, Camille F 
Affiliations: Faculty of Medicine 
Faculty of Medicine 
Faculty of Medicine 
Keywords: Glucose absorption
In-vivo single pass perfusion
Subjects: Colectomy
Issue Date: 2017
Part of: Journal of life sciences
Volume: 174
Start page: 43
End page: 49
Aims The main function of the colon is water and electrolyte absorption. Total colectomy eliminates this colonic function and may alter the absorptive capacity of the small intestine for nutrients. This study examines the effect of total colectomy on jejunal glucose absorption and investigates the potential role of aldosterone in mediating the alterations in glucose uptake post-colectomy using the aldosterone antagonist spironolactone. Main methods Total colectomy with ileo-rectal anastomosis was performed on anesthetized rats. Sham rats were identically handled without colon resection. Two days post-surgery, groups of colectomized rats were injected with either a daily subcutaneous dose of spironolactone or sesame oil for 12 days. Body weight changes and food and water intake were measured in all experimental groups. Glucose absorption was measured by in-vivo single pass perfusion in the rat jejunum of control, sham, colectomized, colectomized with spironolactone, and colectomized with sesame oil treatment. Na/K ATPase, SGK1, SGLT1 and GLUT2 expressions were determined in jejunal mucosa in control, colectomized and colectomized/spironolactone injected rats by Western blot analysis. Histological assessment was performed on jejunal sections in control and colectomized groups. Key findings Glucose absorption significantly increased in colectomized rats with an observed increase in Na/K ATPase and SGK1 expression. No significant expression change in SGLT1 and GLUT2 was detected in the jejunum in colectomized rats. Spironolactone, however, significantly decreased the glucose uptake post-colectomy and normalized Na/K ATPase and SGK1 expression. Significance Our results suggest that jejunal glucose uptake increases post-colectomy as a possible consequence of an aldosterone-mediated function.
DOI: 10.1016/j.lfs.2017.02.012
Ezproxy URL: Link to full text
Type: Journal Article
Appears in Collections:Faculty of Medicine

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