Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/7761
Title: The effect of differentiation, polarization and MOX-LDL treatment on RPS3 and RPL17 expression in THP-1 monocytes and macrophages
Authors: Hamoui, Nouha
Advisors: Nasr, Zeina 
Daher, Jalil 
Keywords: Atherosclerosis, cardiovascular diseases, ribosomal proteins, RPS3, RPL17, M1 macrophages, M2 macrophages, THP-1 monocytes
Subjects: University of Balamand--Dissertations
Dissertations, Academic
Issue Date: 2024
Publisher: [Kalhat, Lebanon] : [University of Balamand], 2024
Abstract: 
Atherosclerosis is an inflammatory disorder marked by the lipid buildup that forms plaques
within artery walls. It is a major worldwide cause of death and a root cause of significant
cardiovascular events like heart attacks and strokes. Macrophages are the main immune cells
that significantly contribute to atherosclerosis onset, progression, and complication. This
involves the ingestion of modified low-density lipoprotein (LDL), like myeloperoxidase
oxidized LDL (Mox-LDL) to form foam cells. Previous studies have implicated various
ribosomal proteins (RPs), such as RPS3 and RPL17, in cell signaling, DNA damage repair,
and apoptosis that are relevant to cardiovascular diseases (CVDs).
This study investigates the impact of differentiation, polarization and Mox-LDL treatment on
the expression of RPS3 and RPL17 in differentiated and polarized macrophages in vitro using
the THP-1 cell line model. This would help expand the body of knowledge concerning the
pathophysiology of atherosclerosis and pave the way for future research into the therapeutic
implications of targeting RPs in CVDs. First, THP-1 monocytes were cultured and
differentiated into M0 macrophages followed by in vitro polarization into M1 and M2
macrophages. Then the monocytes and macrophages were treated with Mox-LDL, after
which the protein expression levels of RPS3 and RPL17 were evaluated through Western blot
analysis. Our results showed that RPS3 portrayed a significant decrease of 5.1-fold in
expression between monocytes and M1 macrophages indicating that polarization had caused
the downregulation. However, RPL17 showed no significant results across all groups, thus
differentiation and polarization did not affect its expression. Finally, Mox-LDL did not
significantly affect the expression of RPS3 and RPL17 in THP-1 monocytes and derived
macrophages. This work might implicate the involvement of RPS3 in macrophage
polarization and provide insights into its potential role in the inflammatory process
underlying atherosclerosis.
Description: 
Includes bibliographical references (p. 42-57)
URI: https://scholarhub.balamand.edu.lb/handle/uob/7761
Rights: This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the personal and educational use exceptions must be obtained from the copyright holder
Type: Thesis
Appears in Collections:UOB Theses and Projects

Show full item record

Record view(s)

11
checked on Feb 17, 2025

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.