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Title: | Virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains isolated from patients with bacteremia | Authors: | Bitar, Joy | Advisors: | Chaar, Mira El | Keywords: | K. pneumoniae, bloodstream infections, classical K. pneumoniae, hypervirulent K. pneumoniae, virulence genes | Subjects: | University of Balamand--Dissertations Dissertations, Academic |
Issue Date: | 2024 | Publisher: | [Kalhat, Lebanon] : [University of Balamand], 2024 | Abstract: | Background: Klebsiella pneumoniae (K. pneumoniae) is a major cause of bloodstream infections (BSI), linked to high morbidity and mortality. Its strains are classified into classical (cKP) and hypervirulent (hvKp) types. The emergence of hypervirulent and multidrug-resistant (MDR) strains, including extended-spectrum β-lactamase (ESBL) and carbapenem-resistant K. pneumoniae (CRKP), presents a significant health threat. This study investigates virulence and resistance profiles of hvKp and cKp strains and genetic diversity between bacteremic and nonbacteremic isolates across Beirut hospitals. Methods: Over 2.5 years (April 2022–August 2024), 62 bacteremic and 32 non-bacteremic K. pneumoniae isolates were collected. Identification used MacConkey, Uri-select, API 20E, and blood agar, with five virulence genes (fimH, entB, mrkD, uge, and wabG) detected by real-time polymerase chain reaction (PCR). Genetic relatedness of six bacteremic isolates was assessed with pulse-field gel electrophoresis (PFGE). Results: Of the bacteremic isolates, one was string test positive (>5 mm). As for the resistance profiles, 50% of the samples exhibited no resistance. However, 35% were classified as ESBL producers, 10% showed CRE, with smaller proportions showing AmpC resistance. Virulence genes were prevalent, but their presence did not significantly differ between bacteremic and nonbacteremic groups. PFGE showed genetic diversity among bacteremic isolates. Conclusion: The prevalence of hypervirulent and multidrug-resistant K. pneumoniae strains underscores the need for enhanced surveillance, antibiotic stewardship, and infection control in Lebanon |
Description: | Includes bibliographical references (p. 55-73) |
URI: | https://scholarhub.balamand.edu.lb/handle/uob/7705 | Rights: | This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the personal and educational use exceptions must be obtained from the copyright holder | Type: | Thesis |
Appears in Collections: | UOB Theses and Projects |
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