Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/7705
Title: Virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains isolated from patients with bacteremia
Authors: Bitar, Joy
Advisors: Chaar, Mira El 
Keywords: K. pneumoniae, bloodstream infections, classical K. pneumoniae, hypervirulent K. pneumoniae, virulence genes
Subjects: University of Balamand--Dissertations
Dissertations, Academic
Issue Date: 2024
Publisher: [Kalhat, Lebanon] : [University of Balamand], 2024
Abstract: 
Background: Klebsiella pneumoniae (K. pneumoniae) is a major cause of bloodstream infections
(BSI), linked to high morbidity and mortality. Its strains are classified into classical (cKP) and
hypervirulent (hvKp) types. The emergence of hypervirulent and multidrug-resistant (MDR)
strains, including extended-spectrum β-lactamase (ESBL) and carbapenem-resistant K.
pneumoniae (CRKP), presents a significant health threat. This study investigates virulence and
resistance profiles of hvKp and cKp strains and genetic diversity between bacteremic and
nonbacteremic isolates across Beirut hospitals.
Methods: Over 2.5 years (April 2022–August 2024), 62 bacteremic and 32 non-bacteremic K.
pneumoniae isolates were collected. Identification used MacConkey, Uri-select, API 20E, and
blood agar, with five virulence genes (fimH, entB, mrkD, uge, and wabG) detected by real-time
polymerase chain reaction (PCR). Genetic relatedness of six bacteremic isolates was assessed
with pulse-field gel electrophoresis (PFGE).
Results: Of the bacteremic isolates, one was string test positive (>5 mm). As for the resistance
profiles, 50% of the samples exhibited no resistance. However, 35% were classified as ESBL
producers, 10% showed CRE, with smaller proportions showing AmpC resistance. Virulence
genes were prevalent, but their presence did not significantly differ between bacteremic and
nonbacteremic groups. PFGE showed genetic diversity among bacteremic isolates.
Conclusion: The prevalence of hypervirulent and multidrug-resistant K. pneumoniae strains
underscores the need for enhanced surveillance, antibiotic stewardship, and infection control in
Lebanon
Description: 
Includes bibliographical references (p. 55-73)
URI: https://scholarhub.balamand.edu.lb/handle/uob/7705
Rights: This object is protected by copyright, and is made available here for research and educational purposes. Permission to reuse, publish, or reproduce the object beyond the personal and educational use exceptions must be obtained from the copyright holder
Type: Thesis
Appears in Collections:UOB Theses and Projects

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