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Title: | Biomolecular evaluation of cryopreserved amniotic membranes for ophthalmological use by ELISA and RT-PCR at one and eighteen months | Authors: | Wahab, C Fakhoury, O Serhan, H Ayash, J Jabbour, F Dirani, A Kallassy, M Waked, N |
Affiliations: | Faculty of Medicine | Keywords: | ARNm Amniotic membrane Cryoconservation Cryopreservation ELISA Elisa Facteurs de croissance Growth factors Membrane amniotique Ophtalmologie Ophthalmology RT-PCR mRNA |
Issue Date: | 2021 | Publisher: | Elsevier | Part of: | Journal Francais d'Ophtalmologie | Volume: | 44 | Issue: | 10 | Start page: | 1529 | End page: | 1535 | Abstract: | To study the presence of certain proteins - EGF (epidermal growth factor), KGF (keratinocyte growth factor), IL-10 (interleukin 10), HGF (hepatocyte growth factor), Alpha2-macroglobulin and IL-1RA (interleukin 1 receptor antagonist) in cryopreserved amniotic membranes at 1 and 18 months and, as a secondary objective, to detect mRNA corresponding to KGF, IL-1Ra, Alpha2-macroglobulin, Fas Ligand, TGF beta (transforming growth factor beta) and Lumican by RT-PCR in membranes preserved at 1 and 18 months. |
URI: | https://scholarhub.balamand.edu.lb/handle/uob/6817 | ISSN: | 01815512 | DOI: | 10.1016/j.jfo.2021.05.013 | Ezproxy URL: | Link to full text | Type: | Journal Article |
Appears in Collections: | Faculty of Medicine |
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