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Title: | Surfactant protein D multimerization and gene polymorphism in COPD and asthma | Authors: | Fakih, Dalia Akiki, Zeina Junker, Kirsten Medlej-Hashim, Myrna Waked, Mirna Salameh, Pascale Holmskov, Uffe Bouharoun-Tayoun, Hasnaa Chamat, Soulaima Sorensen, Grith L Jounblat, Rania |
Affiliations: | Faculty of Medicine | Keywords: | Asthma Chronic obstructive pulmonary disease Multimerization Single nucleotide polymorphism Surfactant protein D |
Issue Date: | 2018 | Publisher: | Wiley Online Library | Part of: | Respirology | Volume: | 23 | Issue: | 3 | Start page: | 298 | End page: | 305 | Abstract: | Background and objective A structural single nucleotide polymorphism rs721917 in the surfactant protein D (SP-D) gene, known as Met11Thr, was reported to influence the circulating levels and degree of multimerization of SP-D and was associated with both COPD and atopy in asthma. Moreover, disease-related processes are known to degrade multimerized SP-D, however, the degree of the protein degradation in these diseases is not clarified. We aimed to determine the distribution of multimerized (high molecular weight (HMW)) and non-multimerized (low molecular weight (LMW)) species of serum SP-D and their correlation with genetic polymorphisms and presence of disease in Lebanese COPD and asthmatic patients. Methods Serum SP-D levels were measured by ELISA in 88 COPD, 121 asthmatic patients and 223 controls. Randomly selected subjects were chosen for genotyping of rs721917 and multimerization studies. HMW and LMW SP-D were separated by gel permeation chromatography. Results Serum SP-D levels were significantly increased in patients with COPD, but not in asthmatic patients, when compared to controls. Met11Thr variation strongly affected serum SP-D levels and the degree of multimerization, but was not associated with COPD and asthma in the study. Remarkably, HMW/LMW serum SP-D ratio was significantly lower in Met11/Met11 COPD and asthmatic patients compared to controls. Conclusion Collectively, non-multimerized species of serum SP-D were dominant in COPD and asthmatic patients suggesting that degradation of SP-D takes place to a significant degree in pulmonary disease. Assays that can separate SP-D proteolytic breakdown products or modified forms from naturally occurring SP-D trimers may result in optimal disease markers for pulmonary inflammatory diseases. |
URI: | https://scholarhub.balamand.edu.lb/handle/uob/5734 | ISSN: | 13237799 | DOI: | 10.1111/resp.13193 | Ezproxy URL: | Link to full text | Type: | Journal Article |
Appears in Collections: | Faculty of Medicine |
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