Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/5299
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dc.contributor.authorAl-Bayssari, Charbelen_US
dc.contributor.authorNawfal Dagher, Taniaen_US
dc.contributor.authorEl Hamoui, Samaren_US
dc.contributor.authorFenianos, Fadien_US
dc.contributor.authorMakdissy, Nehmanen_US
dc.contributor.authorRolain, Jean-Marcen_US
dc.contributor.authorNasreddine, Nadineen_US
dc.date.accessioned2022-01-14T07:36:22Z-
dc.date.available2022-01-14T07:36:22Z-
dc.date.issued2021-
dc.identifier.issn20366590-
dc.identifier.urihttps://scholarhub.balamand.edu.lb/handle/uob/5299-
dc.description.abstractIntroduction: The increasing incidence of infections caused by multidrug-resistant bacteria is considered a global health problem. This study aimed to investigate this resistance in Gram-negative bacteria isolated from patients hospitalized in North-Lebanon. Methodology: All isolates were identified using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antibiotic susceptibility testing was achieved using disk diffusion, E-test and Broth microdilution methods. Phenotypic detection of carbapenemase was carried out using the CarbaNP test. RT-PCR, standard-PCR and sequencing were performed to detect resistance genes and oprD gene. Conjugal transfer was carried out between our isolates and Escherichia coli J53 to detect the genetic localization of resistance genes. MLST was conducted to determine the genotype of each isolate. Results: Twenty-three carbapenem-resistant Enterobacterales of which eight colistin-resistant Escherichia coli, and Twenty carbapenem-resistant Pseudomonas aeruginosa were isolated. All isolates showed an imipenem MIC greater than 32 mg/mL with MICs for colistin greater than 2 mg/L for E. coli isolates. All the Enterobacterales isolates had at least one carbapenemase-encoding gene, with E. coli isolates coharboring blaNDM-4 and mcr-1 genes. Moreover, 16/20 Pseudomonas aeruginosa harbored the blaVIM-2 gene and 18/20 had mutations in the oprD gene. MLST revealed that the isolates belonged to several clones. Conclusions: We report here the first description in the world of clinical E. coli isolates coharboring blaNDM-4 and mcr-1 genes, and K. pneumoniae isolates producing NDM-6 and OXA-48 carbapenemases. Also, we describe the emergence of NDM-1-producing E. cloacae in Lebanon. Screening for these isolates is necessary to limit the spread of resistant microorganisms in hospitals.en_US
dc.language.isoengen_US
dc.subjectLebanonen_US
dc.subjectNDM-4en_US
dc.subjectExtremely drug resistant bacteriaen_US
dc.subjectmcr-1en_US
dc.titleCarbapenem and colistin-resistant bacteria in North Lebanon: Coexistence of mcr-1 and NDM-4 genes in Escherichia colien_US
dc.typeJournal Articleen_US
dc.identifier.doi10.3855/jidc.14176-
dc.identifier.pmid34343118-
dc.identifier.scopus2-s2.0-85112482478-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85112482478-
dc.contributor.affiliationDepartment of Medical Laboratory Sciencesen_US
dc.contributor.affiliationFaculty of Medicineen_US
dc.description.volume15en_US
dc.description.issue7en_US
dc.description.startpage1en_US
dc.description.endpage9en_US
dc.date.catalogued2022-01-14-
dc.description.statusPublisheden_US
dc.identifier.openURLhttps://jidc.org/index.php/journal/article/view/34343118en_US
dc.relation.ispartoftextThe Journal of Infection in Developing Countriesen_US
crisitem.author.parentorgFaculty of Health Sciences-
Appears in Collections:Faculty of Medicine
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