Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/5264
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dc.contributor.authorIsber, Christinaen_US
dc.contributor.authorStockman, David Len_US
dc.contributor.authorDaoud, Ziaden_US
dc.date.accessioned2021-12-16T09:17:39Z-
dc.date.available2021-12-16T09:17:39Z-
dc.date.issued2021-
dc.identifier.urihttps://scholarhub.balamand.edu.lb/handle/uob/5264-
dc.description.abstractThe concept of drug-combination therapy is becoming very important mainly with the drastic increase in resistance to drugs. The Quadruple checkerboard, also called the Q-checkerboard, aims at maximizing the number of possible combinations that can be obtained between four drugs in one experiment to minimize the time and work needed to accomplish the same results with other protocols. This protocol is based on the simple micro dilution technique where the drugs are diluted and combined together in several 96-well plates. In the first set of 96-well plates, Muller-Hinton broth is added followed by the first required drug (e.g., Cefotaxime here) to serially dilute it. After the first step is done, another set of 96-well plates is used to dilute the second drug (e.g., Amikaci), which will be transferred by removing a specific volume of drug 2 and put in the corresponding wells in the first set of 96-well plates that contains drug one. The third step is done by adding the required concentrations of the third drug (e.g., Levofloxacin), to the appropriate plates in the initial set containing combination of drug 1 and 2. The fourth step is done by adding the required concentrations of the fourth drug (e.g., Trimethoprim-sulfamethoxazol) into the appropriate plates in the first set. Then, E. coli ESBL bacterial inoculum will be prepared and added. This method is important to evaluate all the possible combinations and has a wider range of possibilities to be tested furthermore for in vivo testing. Despite being a tiring technique requiring a lot of focus, the results are remarkable and time saving where a lot of combinations can be tested in a single experiment.en_US
dc.language.isoengen_US
dc.titleQuadruple-Checkerboard: A Modification of the Three-Dimensional Checkerboard for Studying Drug Combinationsen_US
dc.typeJournal Articleen_US
dc.identifier.doi10.3791/62311-
dc.identifier.pmid34369930-
dc.identifier.scopus2-s2.0-85114075197-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85114075197-
dc.contributor.affiliationFaculty of Medicineen_US
dc.description.volume2021en_US
dc.description.issue173en_US
dc.date.catalogued2021-12-16-
dc.description.statusPublisheden_US
dc.identifier.openURLhttps://www.jove.com/t/62311/quadruple-checkerboard-modification-three-dimensional-checkerboarden_US
dc.relation.ispartoftextJournal of Visualized Experimenten_US
Appears in Collections:Faculty of Medicine
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