Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/2263
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dc.contributor.authorJekabsons, Mika B.en_US
dc.contributor.authorEchtay, Karimen_US
dc.contributor.authorArechaga, Ignacioen_US
dc.contributor.authorBrand, Martin Den_US
dc.date.accessioned2020-12-23T09:09:45Z-
dc.date.available2020-12-23T09:09:45Z-
dc.date.issued2003-
dc.identifier.urihttps://scholarhub.balamand.edu.lb/handle/uob/2263-
dc.description.abstractne way to study low-abundance mammalian mitochondrial carriers is by ectopically expressing them as bacterial inclusion bodies. Problems encountered with this approach include protein refolding, homogeneity, and stability. In this study, we investigated protein refolding and homogeneity properties of inclusion body human uncoupling protein 2 (UCP2). N-methylanthraniloyl-tagged ATP (Mant-ATP) experiments indicated two independent inclusion body UCP2 binding sites with dissociation constants (Kd) of 0.3–0.5 and 23–92 μM. Dimethylanthranilate, the fluorescent tag without nucleotide, bound with a Kd of greater than 100 μM, suggesting that the low affinity site reflected binding of the tag. By direct titration, UCP2 bound [8-14C] ATP and [8-14C] ADP with Kds of 4–5 and 16–18 μM, respectively. Mg2+ (2 mM) reduced the apparent ATP affinity to 53 μM, an effect entirely explained by chelation of ATP; with Mg2+, Kd using calculated free ATP was 3 μM. A combination of gel filtration, Cu2+-phenanthroline cross-linking, and ultracentrifugation indicated that 75–80% of UCP2 was in a monodisperse, 197 kDa form while the remainder was aggregated. We conclude that (a) Mant-tagged nucleotides are useful fluorescent probes with isolated UCP2 when used with dimethylanthranilate controls; (b) UCP2 binds Mg2+-free nucleotides: the Kd for ATP is about 3–5 μM and for Mant-ATP it is about 10 times lower; and (c) in C12E9 detergent, the monodisperse protein may be in dimeric form.en_US
dc.format.extent10 p.en_US
dc.language.isoengen_US
dc.subjectUCP2en_US
dc.subjectMitochondrial carrieren_US
dc.subjectHydroxyapatiteen_US
dc.subjectFluorescence resonance energy transferen_US
dc.subjectLigand binding (Biochemistry)en_US
dc.subjectGel filtrationen_US
dc.subjectCross-linkingen_US
dc.titleMolecular properties of purified human uncoupling protein 2 refolded from bacterial inclusion bodiesen_US
dc.typeJournal Articleen_US
dc.contributor.affiliationFaculty of Medicineen_US
dc.description.volume35en_US
dc.description.issue5en_US
dc.description.startpage409en_US
dc.description.endpage418en_US
dc.date.catalogued2017-12-14-
dc.description.statusPublisheden_US
dc.identifier.ezproxyURLhttp://ezsecureaccess.balamand.edu.lb/login?url=https://link.springer.com/content/pdf/10.1023%2FA%3A1027335713635.pdfen_US
dc.identifier.OlibID175616-
dc.relation.ispartoftextJournal of bioenergetics and biomembranesen_US
dc.provenance.recordsourceOliben_US
Appears in Collections:Faculty of Medicine
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