Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/2086
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dc.contributor.authorRubino, Stephen Jen_US
dc.contributor.authorMagalhaes, Joao G.en_US
dc.contributor.authorPhilpott, Danaen_US
dc.contributor.authorBahr, George M.en_US
dc.contributor.authorBlanot, Didieren_US
dc.contributor.authorGirardin , Stephen Een_US
dc.date.accessioned2020-12-23T09:06:03Z-
dc.date.available2020-12-23T09:06:03Z-
dc.date.issued2013-
dc.identifier.urihttps://scholarhub.balamand.edu.lb/handle/uob/2086-
dc.description.abstractMuramyl peptides (MPs) represent the building blocks of bacterial peptidoglycan, a critical component of bacterial cell walls. MPs are well characterized for their immunomodulatory properties, and numerous studies have delineated the role of MPs or synthetic MP analogs in host defense, adjuvanticity and inflammation. More recently, Nod1 and Nod2 have been identified as the host sensors for specific MPs, and, in particular, Nod2 was shown to detect muramyl dipeptide (MDP), a MP found in both Gram-positive and Gram-negative bacterial cell walls. Because mutations in Nod2 are associated with the etiology of Crohns disease, there is a need to identify synthetic MP analogs that could potentiate Nod2-dependent immunity. Here, we analyzed the Nod2-activating property of 36 MP analogs that had been tested previously for their adjuvanticity and anti-infectious activity. Using a luciferase-based screen, we demonstrate that addition of a methyl group to the second amino acid of MDP generates a MDP derivative with enhanced Nod2-activating capacity. We further validated these results in murine macrophages, human dendritic cells and in vivo. These results offer a basis for the rational development of synthetic MPs that could be used in the treatment of inflammatory disorders that have been associated with Nod2 dysfunction, such as Crohns disease.en_US
dc.format.extent10 p.en_US
dc.language.isoengen_US
dc.subjectNOD2en_US
dc.subjectMuramyl peptidesen_US
dc.subjectMuramyl dipeptideen_US
dc.subjectAdjuvanten_US
dc.subjectInnate immunityen_US
dc.subject.lcshInflammationen_US
dc.titleIdentification of a synthetic muramyl peptide derivative with enhanced Nod2 stimulatory capacityen_US
dc.typeJournal Articleen_US
dc.identifier.doi10.1177/1753425912471691-
dc.contributor.affiliationFaculty of Medicineen_US
dc.description.volume19en_US
dc.description.issue5en_US
dc.description.startpage439en_US
dc.description.endpage503en_US
dc.date.catalogued2017-11-14-
dc.description.statusPublisheden_US
dc.identifier.OlibID174975-
dc.identifier.openURLhttp://journals.sagepub.com/doi/full/10.1177/1753425912471691en_US
dc.relation.ispartoftextJournal of innate immunityen_US
dc.provenance.recordsourceOliben_US
Appears in Collections:Faculty of Medicine
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