Please use this identifier to cite or link to this item: https://scholarhub.balamand.edu.lb/handle/uob/2078
Title: Human apolipoprotein L1 interferes with mitochondrial function in Saccharomyces cerevisiae
Authors: Chidiac, Mounia
Daher, Jalil 
Boeckstaens, Mélanie
Poelvoorde, Philippe
Badran, Bassam
Marini, Anna Maria
Khalaf, Roy
Vanhamme, Luc
Affiliations: Department of Biology 
Issue Date: 2020
Part of: Molecular medicine reports
Volume: 22
Issue: 3
Start page: 1910
End page: 1920
Abstract: 
To the best of our knowledge, the vertebrate apolipoprotein L (APOL) family has not previously been ascribed to any definite pathophysiological function, although the conserved BH3 protein domain suggests a role in programmed cell death or an interference with mitochondrial processes. In the present study, the human APOL1 was expressed in the yeast Saccharomyces cerevisiae in order to determine the molecular action of APOL1. APOL1 inhibited cell proliferation in a non‑fermentable carbon source, such as glycerol, while it had no effect on proliferation in fermentable carbon sources, such as galactose. APOL1, expressed in yeast, is localized in the mitochondrial fraction, as determined via western blotting. APOL1 induced a loss of mitochondrial function, demonstrated by a loss of respiratory index, and mitochondrial membrane potential. Green fluorescent protein tagging of mitochondrial protein revealed that APOL1 was associated with abnormal mitochondrial and lysosomal morphologies, observed by a loss of the normal mitochondrial tubular network. Thus, the results of the present study suggest that APOL1 could be a physiological regulator of mitochondrial function.
URI: https://scholarhub.balamand.edu.lb/handle/uob/2078
DOI: 10.3892/mmr.2020.11271
Open URL: Link to full text
Type: Journal Article
Appears in Collections:Department of Biology

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